4pcv: Difference between revisions
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==The structure of BdcA (YjgI) from E. coli== | ==The structure of BdcA (YjgI) from E. coli== | ||
<StructureSection load='4pcv' size='340' side='right' caption='[[4pcv]], [[Resolution|resolution]] 2.05Å' scene=''> | <StructureSection load='4pcv' size='340' side='right' caption='[[4pcv]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4pcv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCV FirstGlance]. <br> | <table><tr><td colspan='2'>[[4pcv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCV FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pcv RCSB], [http://www.ebi.ac.uk/pdbsum/4pcv PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcv OCA], [http://pdbe.org/4pcv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pcv RCSB], [http://www.ebi.ac.uk/pdbsum/4pcv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4pcv" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:12, 4 January 2017
The structure of BdcA (YjgI) from E. coliThe structure of BdcA (YjgI) from E. coli
Structural highlights
Publication Abstract from PubMedThe Escherichia coli protein BdcA (previously referred to as YjgI) plays a key role in the dispersal of cells from bacterial biofilms, and its constitutive activation provides an attractive therapeutic target for dismantling these communities. In order to investigate the function of BdcA at a molecular level, we integrated structural and functional studies. Our 2.05 A structure of BdcA shows that it is a member of the NAD(P)(H)-dependent short-chain dehydrogenase/reductase (SDR) superfamily. Structural comparisons with other members of the SDR family suggested that BdcA binds NADP(H). This was demonstrated experimentally using thermal denaturation studies, which showed that BcdA binds specifically to NADPH. Subsequent ITC experiments further confirmed this result and reported a Kd of 25.9 microM. Thus, BdcA represents the newest member of the limited number of oxidoreductases shown to be involved in quorum sensing and biofilm dispersal. BdcA, a protein important for Escherichia coli biofilm dispersal, is a short-chain dehydrogenase/reductase that binds specifically to NADPH.,Lord DM, Baran AU, Wood TK, Peti W, Page R PLoS One. 2014 Sep 22;9(9):e105751. doi: 10.1371/journal.pone.0105751., eCollection 2014. PMID:25244619[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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