1ree: Difference between revisions
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|PDB= 1ree |SIZE=350|CAPTION= <scene name='initialview01'>1ree</scene>, resolution 1.6Å | |PDB= 1ree |SIZE=350|CAPTION= <scene name='initialview01'>1ree</scene>, resolution 1.6Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=C4X:3,4-EPOXYBUTYL-BETA-D-XYLOSIDE'>C4X</scene> | |LIGAND= <scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=C4X:3,4-EPOXYBUTYL-BETA-D-XYLOSIDE'>C4X</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ree FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ree OCA], [http://www.ebi.ac.uk/pdbsum/1ree PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ree RCSB]</span> | |||
}} | }} | ||
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[[Category: Rouvinen, J.]] | [[Category: Rouvinen, J.]] | ||
[[Category: Torronen, A.]] | [[Category: Torronen, A.]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: xylan degradation]] | [[Category: xylan degradation]] | ||
[[Category: xylanase]] | [[Category: xylanase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:26:58 2008'' | ||
Revision as of 23:26, 30 March 2008
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| , resolution 1.6Å | |||||||
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| Ligands: | , , | ||||||
| Activity: | Endo-1,4-beta-xylanase, with EC number 3.2.1.8 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 3,4-EPOXYBUTYL-BETA-D-XYLOSIDE
OverviewOverview
The three-dimensional structures of endo-1,4-xylanase II (XYNII) from Trichoderma reesei complexed with 4,5-epoxypentyl beta-D-xyloside (X-O-C5),3,4-epoxybutyl beta-D-xyloside (X-O-C4), and 2,3-epoxypropyl beta-D-xyloside (X-O-C3) were determined by X-ray crystallography. High-resolution measurement revealed clear electron densities for each ligand. Both X-O-C5 and X-O-C3 were found to form a covalent bond with the putative nucleophile Glu86. Unexpectedly, X-O-C4 was found to bind to the putative acid/base catalyst Glu177. In all three complexes, clear conformational changes were found in XYNII compared to the native structure. These changes were largest in the X-O-C3 complex structure.
About this StructureAbout this Structure
1REE is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.
ReferenceReference
Covalent binding of three epoxyalkyl xylosides to the active site of endo-1,4-xylanase II from Trichoderma reesei., Havukainen R, Torronen A, Laitinen T, Rouvinen J, Biochemistry. 1996 Jul 23;35(29):9617-24. PMID:8755744
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