1rb7: Difference between revisions
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|PDB= 1rb7 |SIZE=350|CAPTION= <scene name='initialview01'>1rb7</scene>, resolution 2.10Å | |PDB= 1rb7 |SIZE=350|CAPTION= <scene name='initialview01'>1rb7</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] </span> | ||
|GENE= FCY1, YPR062W, YP9499.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= FCY1, YPR062W, YP9499.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1ox7|1OX7]], [[1p6o|1P6O]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rb7 OCA], [http://www.ebi.ac.uk/pdbsum/1rb7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rb7 RCSB]</span> | |||
}} | }} | ||
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[[Category: Ireton, G C.]] | [[Category: Ireton, G C.]] | ||
[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
[[Category: amino hydrolase]] | [[Category: amino hydrolase]] | ||
[[Category: cytosine deaminase]] | [[Category: cytosine deaminase]] | ||
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[[Category: zinc bound]] | [[Category: zinc bound]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:44 2008'' | ||
Revision as of 23:25, 30 March 2008
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| , resolution 2.10Å | |||||||
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| Ligands: | |||||||
| Gene: | FCY1, YPR062W, YP9499.17 (Saccharomyces cerevisiae) | ||||||
| Activity: | Cytosine deaminase, with EC number 3.5.4.1 | ||||||
| Related: | 1OX7, 1P6O
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Yeast cytosine deaminase crystal form p212121 with sodium acetate.
OverviewOverview
A crystallization strategy termed 'microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible. This method is an extension of conventional seeding techniques in which microseeds from the nucleation step are systematically seeded into new conditions where all components of the drop are allowed to vary to screen for subsequent growth of well ordered specimens. The structure of a crystal form of yeast cytosine deaminase produced by streak-seeding using a single condition for both nucleation and growth is compared with the structure of a related crystal form produced by separating nucleation and growth conditions. The resulting structural comparison demonstrates that differential chelation patterns of cations by acidic surface residues of proteins within crystal lattice contacts is a critical parameter of crystal nucleation and growth.
About this StructureAbout this Structure
1RB7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Microseed matrix screening to improve crystals of yeast cytosine deaminase., Ireton GC, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):601-5. Epub 2004, Feb 25. PMID:14993707
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