5md0: Difference between revisions
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The | ==The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=5, twist=6== | ||
<StructureSection load='5md0' size='340' side='right' caption='[[5md0]], [[Resolution|resolution]] 8.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5md0]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MD0 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5md0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5md0 OCA], [http://pdbe.org/5md0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5md0 RCSB], [http://www.ebi.ac.uk/pdbsum/5md0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5md0 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
HIV-1 contains a cone-shaped capsid encasing the viral genome. This capsid is thought to follow fullerene geometry-a curved hexameric lattice of the capsid protein, CA, closed by incorporating 12 CA pentamers. Current models for core structure are based on crystallography of hexameric and cross-linked pentameric CA, electron microscopy of tubular CA arrays, and simulations. Here, we report subnanometer-resolution cryo-electron tomography structures of hexameric and pentameric CA within intact HIV-1 particles. Whereas the hexamer structure is compatible with crystallography studies, the pentamer forms using different interfaces. Determining multiple structures revealed how CA flexes to form the variably curved core shell. We show that HIV-1 CA assembles both aberrant and perfect fullerene cones, supporting models in which conical cores assemble de novo after maturation. | |||
The structure and flexibility of conical HIV-1 capsids determined within intact virions.,Mattei S, Glass B, Hagen WJ, Krausslich HG, Briggs JA Science. 2016 Dec 16;354(6318):1434-1437. PMID:27980210<ref>PMID:27980210</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5md0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human immunodeficiency virus 1]] | |||
[[Category: Briggs, J A.G]] | |||
[[Category: Glass, B]] | [[Category: Glass, B]] | ||
[[Category: Hagen, W | [[Category: Hagen, W J.H]] | ||
[[Category: Kraeusslich, H G]] | |||
[[Category: Mattei, S]] | [[Category: Mattei, S]] | ||
[[Category: | [[Category: Capsid]] | ||
[[Category: | [[Category: Hiv-1]] | ||
[[Category: Lattice curvature]] | |||
[[Category: Retrovirus]] | |||
[[Category: Viral protein]] | |||
Revision as of 19:18, 2 January 2017
The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=5, twist=6The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=5, twist=6
Structural highlights
Publication Abstract from PubMedHIV-1 contains a cone-shaped capsid encasing the viral genome. This capsid is thought to follow fullerene geometry-a curved hexameric lattice of the capsid protein, CA, closed by incorporating 12 CA pentamers. Current models for core structure are based on crystallography of hexameric and cross-linked pentameric CA, electron microscopy of tubular CA arrays, and simulations. Here, we report subnanometer-resolution cryo-electron tomography structures of hexameric and pentameric CA within intact HIV-1 particles. Whereas the hexamer structure is compatible with crystallography studies, the pentamer forms using different interfaces. Determining multiple structures revealed how CA flexes to form the variably curved core shell. We show that HIV-1 CA assembles both aberrant and perfect fullerene cones, supporting models in which conical cores assemble de novo after maturation. The structure and flexibility of conical HIV-1 capsids determined within intact virions.,Mattei S, Glass B, Hagen WJ, Krausslich HG, Briggs JA Science. 2016 Dec 16;354(6318):1434-1437. PMID:27980210[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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