4hgu: Difference between revisions
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==Crystal Structure of Galleria mellonella Silk Protease Inhibitor 2== | ==Crystal Structure of Galleria mellonella Silk Protease Inhibitor 2== | ||
<StructureSection load='4hgu' size='340' side='right' caption='[[4hgu]], [[Resolution|resolution]] 0.98Å' scene=''> | <StructureSection load='4hgu' size='340' side='right' caption='[[4hgu]], [[Resolution|resolution]] 0.98Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1an1|1an1]], [[2ovo|2ovo]], [[3pis|3pis]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1an1|1an1]], [[2ovo|2ovo]], [[3pis|3pis]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgu RCSB], [http://www.ebi.ac.uk/pdbsum/4hgu PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgu OCA], [http://pdbe.org/4hgu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hgu RCSB], [http://www.ebi.ac.uk/pdbsum/4hgu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4hgu" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 22:54, 15 December 2016
Crystal Structure of Galleria mellonella Silk Protease Inhibitor 2Crystal Structure of Galleria mellonella Silk Protease Inhibitor 2
Structural highlights
Publication Abstract from PubMedPurification of suitable quantity of homogenous protein is very often the bottleneck in protein structural studies. Overexpression of a desired gene and attachment of enzymatically cleavable affinity tags to the protein of interest made a breakthrough in this field. Here we describe the structure of Galleria mellonella silk proteinase inhibitor 2 (GmSPI-2) determined both by X-ray diffraction and NMR spectroscopy methods. GmSPI-2 was purified using a new method consisting in non-enzymatic His-tag removal based on a highly specific peptide bond cleavage reaction assisted by Ni(II) ions. The X-ray crystal structure of GmSPI-2 was refined against diffraction data extending to 0.98 A resolution measured at 100 K using synchrotron radiation. Anisotropic refinement with the removal of stereochemical restraints for the well-ordered parts of the structure converged with R factor of 10.57% and Rfree of 12.91%. The 3D structure of GmSPI-2 protein in solution was solved on the basis of 503 distance constraints, 10 hydrogen bonds and 26 torsion angle restraints. It exhibits good geometry and side-chain packing parameters. The models of the protein structure obtained by X-ray diffraction and NMR spectroscopy are very similar to each other and reveal the same beta2alphabeta fold characteristic for Kazal-family serine proteinase inhibitors. Atomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor.,Kopera E, Bal W, Lenarcic Zivkovic M, Dvornyk A, Kludkiewicz B, Grzelak K, Zhukov I, Zagorski-Ostoja W, Jaskolski M, Krzywda S PLoS One. 2014 Sep 18;9(9):e106936. doi: 10.1371/journal.pone.0106936., eCollection 2014. PMID:25233114[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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