1qoq: Difference between revisions
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|PDB= 1qoq |SIZE=350|CAPTION= <scene name='initialview01'>1qoq</scene>, resolution 1.8Å | |PDB= 1qoq |SIZE=350|CAPTION= <scene name='initialview01'>1qoq</scene>, resolution 1.8Å | ||
|SITE= <scene name='pdbsite=IGP:Plp+Binding+Site+For+Chain+B'>IGP</scene> | |SITE= <scene name='pdbsite=IGP:Plp+Binding+Site+For+Chain+B'>IGP</scene> | ||
|LIGAND= <scene name='pdbligand=IGP:INDOLE-3-GLYCEROL+PHOSPHATE'>IGP</scene> | |LIGAND= <scene name='pdbligand=IGP:INDOLE-3-GLYCEROL+PHOSPHATE'>IGP</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span> | ||
|GENE= TRPA/TRPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | |GENE= TRPA/TRPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2wsy|2WSY]], [[1ttp|1TTP]], [[1ttq|1TTQ]], [[2trs|2TRS]], [[2tsy|2TSY]], [[1ubs|1UBS]], [[1a5a|1A5A]], [[1a5b|1A5B]], [[1a5s|1A5S]], [[1a50|1A50]], [[1beu|1BEU]], [[1bks|1BKS]], [[1qop|1QOP]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qoq OCA], [http://www.ebi.ac.uk/pdbsum/1qoq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qoq RCSB]</span> | |||
}} | }} | ||
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[[Category: Schlichting, I.]] | [[Category: Schlichting, I.]] | ||
[[Category: Weyand, M.]] | [[Category: Weyand, M.]] | ||
[[Category: carbon-oxygen lyase]] | [[Category: carbon-oxygen lyase]] | ||
[[Category: lyase]] | [[Category: lyase]] | ||
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[[Category: tryptophan biosynthesis]] | [[Category: tryptophan biosynthesis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:16:49 2008'' | ||
Revision as of 23:16, 30 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , | ||||||
| Gene: | TRPA/TRPB (Salmonella typhimurium) | ||||||
| Activity: | Tryptophan synthase, with EC number 4.2.1.20 | ||||||
| Related: | 2WSY, 1TTP, 1TTQ, 2TRS, 2TSY, 1UBS, 1A5A, 1A5B, 1A5S, 1A50, 1BEU, 1BKS, 1QOP
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH INDOLE GLYCEROL PHOSPHATE
OverviewOverview
We used freeze trapping to stabilize the Michaelis complex of wild-type tryptophan synthase and the alpha-subunit substrate indole-3-glycerol phosphate (IGP) and determined its structure to 1. 8 A resolution. In addition, we determined the 1.4 A resolution structure of the complex with indole-3-propanole phosphate (IPP), a noncleavable IGP analogue. The interaction of the 3'-hydroxyl of IGP with the catalytic alphaGlu49 leads to a twisting of the propane chain and to a repositioning of the indole ring compared to IPP. Concomitantly, the catalytic alphaAsp60 rotates resulting in a translocation of the COMM domain [betaGly102-betaGly189, for definition see Schneider et al. (1998) Biochemistry 37, 5394-5406] in a direction opposite to the one in the IPP complex. This results in loss of the allosteric sodium ion bound at the beta-subunit and an opening of the beta-active site, thereby making the cofactor pyridoxal 5'-phosphate (PLP) accessible to solvent and thus serine binding. These findings form the structural basis for the information transfer from the alpha- to the beta-subunit and may explain the affinity increase of the beta-active site for serine upon IGP binding.
About this StructureAbout this Structure
1QOQ is a Protein complex structure of sequences from Salmonella typhimurium. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of wild-type tryptophan synthase complexed with the natural substrate indole-3-glycerol phosphate., Weyand M, Schlichting I, Biochemistry. 1999 Dec 14;38(50):16469-80. PMID:10600108
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