1qjb: Difference between revisions
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|PDB= 1qjb |SIZE=350|CAPTION= <scene name='initialview01'>1qjb</scene>, resolution 2.0Å | |PDB= 1qjb |SIZE=350|CAPTION= <scene name='initialview01'>1qjb</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qjb OCA], [http://www.ebi.ac.uk/pdbsum/1qjb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qjb RCSB]</span> | |||
}} | }} | ||
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[[Category: signal transducti]] | [[Category: signal transducti]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:14:25 2008'' | ||
Revision as of 23:14, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)
OverviewOverview
We have solved the high-resolution X-ray structure of 14-3-3 bound to two different phosphoserine peptides, representing alternative substrate-binding motifs. These structures reveal an evolutionarily conserved network of peptide-protein interactions within all 14-3-3 isotypes, explain both binding motifs, and identify a novel intrachain phosphorylation-mediated loop structure in one of the peptides. A 14-3-3 mutation disrupting Raf signaling alters the ligand-binding cleft, selecting a different phosphopeptide-binding motif and different substrates than the wild-type protein. Many 14-3-3: peptide contacts involve a C-terminal amphipathic alpha helix containing a putative nuclear export signal, implicating this segment in both ligand and Crm1 binding. Structural homology between the 14-3-3 NES structure and those within I kappa B alpha and p53 reveals a conserved topology recognized by the Crm1 nuclear export machinery.
About this StructureAbout this Structure
1QJB is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 14PS. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of 14-3-3 phosphopeptide complexes identifies a dual role for the nuclear export signal of 14-3-3 in ligand binding., Rittinger K, Budman J, Xu J, Volinia S, Cantley LC, Smerdon SJ, Gamblin SJ, Yaffe MB, Mol Cell. 1999 Aug;4(2):153-66. PMID:10488331
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