1q90: Difference between revisions

Revision as of 23:10, 30 March 2008

, resolution 3.10Å

Ligands:

, , , , , , ,

Gene:

PETA (Chlamydomonas reinhardtii), PETB (Chlamydomonas reinhardtii), PETC (Chlamydomonas reinhardtii), PETD (Chlamydomonas reinhardtii), PETG (Chlamydomonas reinhardtii), PETL (Chlamydomonas reinhardtii), PETM (Chlamydomonas reinhardtii), PETN (Chlamydomonas reinhardtii)

Activity:

Plastoquinol--plastocyanin reductase, with EC number 1.10.99.1

Resources:

FirstGlance, OCA, PDBsum, RCSB

Coordinates:

save as pdb, mmCIF, xml

Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii

OverviewOverview

Photosystems I and II (PSI and II) are reaction centres that capture light energy in order to drive oxygenic photosynthesis; however, they can only do so by interacting with the multisubunit cytochrome b(6)f complex. This complex receives electrons from PSII and passes them to PSI, pumping protons across the membrane and powering the Q-cycle. Unlike the mitochondrial and bacterial homologue cytochrome bc(1), cytochrome b(6)f can switch to a cyclic mode of electron transfer around PSI using an unknown pathway. Here we present the X-ray structure at 3.1 A of cytochrome b(6)f from the alga Chlamydomonas reinhardtii. The structure bears similarities to cytochrome bc(1) but also exhibits some unique features, such as binding chlorophyll, beta-carotene and an unexpected haem sharing a quinone site. This haem is atypical as it is covalently bound by one thioether linkage and has no axial amino acid ligand. This haem may be the missing link in oxygenic photosynthesis.

About this StructureAbout this Structure

1Q90 is a Protein complex structure of sequences from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

ReferenceReference

An atypical haem in the cytochrome b(6)f complex., Stroebel D, Choquet Y, Popot JL, Picot D, Nature. 2003 Nov 27;426(6965):413-8. PMID:14647374

Page seeded by OCA on Sun Mar 30 23:10:27 2008

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OCA

@@ Line 4: / Line 4: @@
 |PDB= 1q90 |SIZE=350|CAPTION= <scene name='initialview01'>1q90</scene>, resolution 3.10&Aring;
 |SITE=
-|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=CL1:ALPHA+CHLOROPHYLL+A'>CL1</scene>, <scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=TDS:8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE'>TDS</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene> and <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>
+|LIGAND= <scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=CL1:ALPHA+CHLOROPHYLL+A'>CL1</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene>, <scene name='pdbligand=TDS:8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE'>TDS</scene>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Plastoquinol--plastocyanin_reductase Plastoquinol--plastocyanin reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.99.1 1.10.99.1]
+|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Plastoquinol--plastocyanin_reductase Plastoquinol--plastocyanin reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.99.1 1.10.99.1] </span>
 |GENE= PETA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii]), PETN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 Chlamydomonas reinhardtii])
+|DOMAIN=
+|RELATEDENTRY=
+|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q90 OCA], [http://www.ebi.ac.uk/pdbsum/1q90 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q90 RCSB]</span>
 }}
@@ Line 27: / Line 30: @@
 [[Category: Popot, J L.]]
 [[Category: Stroebel, D.]]
-[[Category: BCR]]
-[[Category: CL1]]
-[[Category: FES]]
-[[Category: HEM]]
-[[Category: LFA]]
-[[Category: LMG]]
-[[Category: SQD]]
-[[Category: TDS]]
 [[Category: beta-carotene]]
 [[Category: chlorophyll]]
@@ Line 45: / Line 40: @@
 [[Category: sulfoquinovosyldiacylglycerol]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:34:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:10:27 2008''