1pev: Difference between revisions

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Revision as of 22:58, 30 March 2008

File:1pev.gif

, resolution 2.00Å

Gene:

UNC-78 OR C04F6.4 (Caenorhabditis elegans)

Domains:

WD40, COG2319

Related:

1NR0

Resources:

FirstGlance, OCA, PDBsum, RCSB

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of the Actin Interacting Protein from Caenorhabditis Elegans

OverviewOverview

Actin-interacting protein 1 (AIP1) is a WD40 repeat protein that enhances actin filament disassembly in the presence of actin-depolymerizing factor (ADF)/cofilin. AIP1 also caps the barbed end of ADF/cofilin-bound actin filament. However, the mechanism by which AIP1 interacts with ADF/cofilin and actin is not clearly understood. We determined the crystal structure of Caenorhabditis elegans AIP1 (UNC-78), which revealed 14 WD40 modules arranged in two seven-bladed beta-propeller domains. The structure allowed for the mapping of conserved surface residues, and mutagenesis studies identified five residues that affected the ADF/cofilin-dependent actin filament disassembly activity. Mutations of these residues, which reside in blades 3 and 4 in the N-terminal propeller domain, had significant effects on the disassembly activity but did not alter the barbed end capping activity. These data support a model in which this conserved surface of AIP1 plays a direct role in enhancing fragmentation/depolymerization of ADF/cofilin-bound actin filaments but not in barbed end capping.

About this StructureAbout this Structure

1PEV is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

ReferenceReference

Identification of functional residues on Caenorhabditis elegans actin-interacting protein 1 (UNC-78) for disassembly of actin depolymerizing factor/cofilin-bound actin filaments., Mohri K, Vorobiev S, Fedorov AA, Almo SC, Ono S, J Biol Chem. 2004 Jul 23;279(30):31697-707. Epub 2004 May 18. PMID:15150269

Page seeded by OCA on Sun Mar 30 22:58:42 2008

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OCA

@@ Line 8: / Line 8: @@
 |GENE= UNC-78 OR C04F6.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])
 |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00200 WD40], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2319 COG2319]</span>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pev OCA], [http://www.ebi.ac.uk/pdbsum/1pev PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1pev RCSB]</span>
+|RELATEDENTRY=[[1nr0|1NR0]]
+|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pev OCA], [http://www.ebi.ac.uk/pdbsum/1pev PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pev RCSB]</span>
 }}
@@ Line 42: / Line 43: @@
 [[Category: wd40 repeat]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:00:35 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:58:42 2008''