1p9r: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 1p9r |SIZE=350|CAPTION= <scene name='initialview01'>1p9r</scene>, resolution 2.50Å | |PDB= 1p9r |SIZE=350|CAPTION= <scene name='initialview01'>1p9r</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae]) | |GENE= EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1p9r|1P9R]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9r OCA], [http://www.ebi.ac.uk/pdbsum/1p9r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p9r RCSB]</span> | |||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Robien, M A.]] | [[Category: Robien, M A.]] | ||
[[Category: Sandkvist, M.]] | [[Category: Sandkvist, M.]] | ||
[[Category: bacterial type ii secretion system cytoplasmic protein - gspe]] | [[Category: bacterial type ii secretion system cytoplasmic protein - gspe]] | ||
[[Category: metalloprotein (metal-cys4 site)]] | [[Category: metalloprotein (metal-cys4 site)]] | ||
[[Category: putative atpase/ atp binding protein]] | [[Category: putative atpase/ atp binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:56:56 2008'' | ||
Revision as of 22:56, 30 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | EPSE OR VC2732 (Vibrio cholerae) | ||||||
| Related: | 1P9R
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Vibrio cholerae putative NTPase EpsE
OverviewOverview
Type II secretion systems consist of an assembly of 12-15 Gsp proteins responsible for transporting a variety of virulence factors across the outer membrane in several pathogenic bacteria. In Vibrio cholerae, the major virulence factor cholera toxin is secreted by the Eps Type II secretion apparatus consisting of 14 Eps proteins. One of these, EpsE, is a cytoplasmic putative NTPase essential for the functioning of the Eps system and member of the GspE subfamily of Type II secretion ATPases. The crystal structure of a truncated form of EpsE in nucleotide-liganded and unliganded state has been determined, and reveals a two-domain architecture with the four characteristic sequence "boxes" of the GspE subfamily clustering around the nucleotide-binding site of the C-domain. This domain contains two C-terminal subdomains not reported before in this superfamily of NTPases. One of these subdomains contains a four-cysteine motif that appears to be involved in metal binding as revealed by anomalous difference density. The EpsE subunits form a right-handed helical arrangement in the crystal with extensive and conserved contacts between the C and N domains of neighboring subunits. Combining the most conserved interface with the quaternary structure of the C domain in a distant homolog, a hexameric model for EpsE is proposed which may reflect the assembly of this critical protein in the Type II secretion system. The nucleotide ligand contacts both domains in this model. The N2-domain-containing surface of the hexamer appears to be highly conserved in the GspE family and most likely faces the inner membrane interacting with other members of the Eps system.
About this StructureAbout this Structure
1P9R is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae., Robien MA, Krumm BE, Sandkvist M, Hol WG, J Mol Biol. 2003 Oct 24;333(3):657-74. PMID:14556751
Page seeded by OCA on Sun Mar 30 22:56:56 2008