1p3y: Difference between revisions
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|PDB= 1p3y |SIZE=350|CAPTION= <scene name='initialview01'>1p3y</scene>, resolution 2.54Å | |PDB= 1p3y |SIZE=350|CAPTION= <scene name='initialview01'>1p3y</scene>, resolution 2.54Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= mrsD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1409 Bacillus sp.]) | |GENE= mrsD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1409 Bacillus sp.]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p3y OCA], [http://www.ebi.ac.uk/pdbsum/1p3y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p3y RCSB]</span> | |||
}} | }} | ||
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[[Category: Kupke, T.]] | [[Category: Kupke, T.]] | ||
[[Category: Steinbacher, S.]] | [[Category: Steinbacher, S.]] | ||
[[Category: cystein]] | [[Category: cystein]] | ||
[[Category: flavoprotein]] | [[Category: flavoprotein]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:54:29 2008'' | ||
Revision as of 22:54, 30 March 2008
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| , resolution 2.54Å | |||||||
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| Ligands: | |||||||
| Gene: | mrsD (Bacillus sp.) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MrsD from Bacillus sp. HIL-Y85/54728
OverviewOverview
MrsD from Bacillus sp. HIL-Y85/54728 is a member of the HFCD (homo-oligomeric flavin-containing Cys decarboxylases) family of flavoproteins and is involved in the biosynthesis of the lantibiotic mersacidin. It catalyses the oxidative decarboxylation of the C-terminal cysteine residue of the MrsA precursor peptide of mersacidin, yielding a (Z)-enethiol intermediate as the first step in the formation of the unusual amino acid S-[(Z)-2-aminovinyl]-methyl-D-cysteine. Surprisingly, MrsD was found to bind FAD, in contrast to the three other characterized members of the HFCD family, which bind FMN. To determine the molecular discriminators of FAD binding within the HFCD family, the crystal structure of MrsD was analyzed at a resolution of 2.54 A. Crystals of space group F432 contain one MrsD monomer in the asymmetric unit. However, a Patterson search with EpiD-derived models failed. Based on the consideration that the dodecameric MrsD particle of tetrahedral symmetry resembles the quaternary structure of EpiD, rotational and translational parameters were derived from the geometric consideration that the MrsD dodecamer is generated from a monomer by crystallographic symmetry around the position (1/4, 1/4, 1/4) of the unit cell. A structural comparison with the FMN-binding members of the HFCD family EpiD and AtHAL3a shows conserved sequence motifs in contact with the flavin's pyrimidine ring but divergent environments for the dimethylbenzene ring of the isoalloxazine moiety. The position of the ribityl chain differs in MrsD from that found in EpiD and AtHAL3a. However, the FMN-phosphate binding sites are also highly conserved in their exact positions. In all three cases, the flavin cofactor is bound to a structurally conserved region of the Rossmann-fold monomer, exposing its Re side for catalysis. The adenosyl phosphate of FAD is anchored in a well defined binding site and the adenosine moieties are oriented towards the interior of the hollow particle, where three of them pack against each other around the threefold axis of a trimeric facet.
About this StructureAbout this Structure
1P3Y is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.
ReferenceReference
Structure of MrsD, an FAD-binding protein of the HFCD family., Blaesse M, Kupke T, Huber R, Steinbacher S, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1414-21. Epub 2003, Jul 23. PMID:12876343
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