CRISPR-Cas9: Difference between revisions
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<scene name='74/742625/Cv/34'>Cas5 caps the tail of the Cas7 filament at the 5′ end of the crRNA</scene>. The structure of Cascade reveals that Cas5 is structurally related to Cas7, as it consists of a palm(residues 1 to 78 and 115 to 224) and a thumb (residues 79 to 114) domain, but lacks a fingers domain. <scene name='74/742625/Cv1/3'>Rocket representation of Cas7 and Cas5 colored by domain: thumb (green), fingers (blue), and palm (purple)</scene>. Close-up view of the <scene name='74/742625/Cv/35'>interaction between Cas5, Cas7.6, and the 5′ hook</scene>. | <scene name='74/742625/Cv/34'>Cas5 caps the tail of the Cas7 filament at the 5′ end of the crRNA</scene>. The structure of Cascade reveals that Cas5 is structurally related to Cas7, as it consists of a palm(residues 1 to 78 and 115 to 224) and a thumb (residues 79 to 114) domain, but lacks a fingers domain. <scene name='74/742625/Cv1/3'>Rocket representation of Cas7 and Cas5 colored by domain: thumb (green), fingers (blue), and palm (purple)</scene>. Close-up view of the <scene name='74/742625/Cv/35'>interaction between Cas5, Cas7.6, and the 5′ hook</scene>. | ||
==Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target | ==Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target at 2.45 angstrom resolution<ref>PMID:26863189</ref>== | ||
<scene name='74/742625/Cv2/4'>Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target</scene>. | <scene name='74/742625/Cv2/4'>Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target</scene>. | ||