CRISPR-Cas9: Difference between revisions
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===Stabilization of the guide-target hybrid by Cas7, Cse1, and Cse2.=== | ===Stabilization of the guide-target hybrid by Cas7, Cse1, and Cse2.=== | ||
<scene name='74/742625/Cv/26'>Each 5-bp segment of the hybrid is situated between the palm of one Cas7 subunit (e.g. Cas7.4) and the fingers of the adjacent subunit (e.g. Cas7.5)</scene>. 5-bp segment is colored red. | <scene name='74/742625/Cv/26'>Each 5-bp segment of the hybrid is situated between the palm of one Cas7 subunit (e.g. Cas7.4) and the fingers of the adjacent subunit (e.g. Cas7.5)</scene>. 5-bp segment is colored red. Extensive contacts between the guide region of the crRNA and the Cas7 filament bury a large portion of the crRNA backbone, leaving the bases solvent-exposed. The absence of direct contacts between protein side chains and bases of the crRNA explains the lack of sequence specificity by Cascade for the guide sequence. <scene name='74/742625/Cv/27'>Close-up view of the bound crRNA</scene>. The DNA target has been removed for clarity. Intercalation by Met166 from Cas7 is highlighted. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||