1oqc: Difference between revisions
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|PDB= 1oqc |SIZE=350|CAPTION= <scene name='initialview01'>1oqc</scene>, resolution 1.80Å | |PDB= 1oqc |SIZE=350|CAPTION= <scene name='initialview01'>1oqc</scene>, resolution 1.80Å | ||
|SITE= <scene name='pdbsite=CTA:Putative+Catalytic+Site'>CTA</scene>, <scene name='pdbsite=CTB:Putative+Catalytic+Site'>CTB</scene>, <scene name='pdbsite=CTC:Putative+Catalytic+Site'>CTC</scene> and <scene name='pdbsite=CTD:Putative+Catalytic+Site'>CTD</scene> | |SITE= <scene name='pdbsite=CTA:Putative+Catalytic+Site'>CTA</scene>, <scene name='pdbsite=CTB:Putative+Catalytic+Site'>CTB</scene>, <scene name='pdbsite=CTC:Putative+Catalytic+Site'>CTC</scene> and <scene name='pdbsite=CTD:Putative+Catalytic+Site'>CTD</scene> | ||
|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ALR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |GENE= ALR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oqc OCA], [http://www.ebi.ac.uk/pdbsum/1oqc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oqc RCSB]</span> | |||
}} | }} | ||
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[[Category: Wang, B C.]] | [[Category: Wang, B C.]] | ||
[[Category: Wu, C K.]] | [[Category: Wu, C K.]] | ||
[[Category: alr]] | [[Category: alr]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
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[[Category: sulfhydryl oxidase]] | [[Category: sulfhydryl oxidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:48:48 2008'' | ||
Revision as of 22:48, 30 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , and | ||||||
| Ligands: | |||||||
| Gene: | ALR (Rattus norvegicus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of augmenter of liver regeneration: a mammalian FAD dependent sulfhydryl oxidase
OverviewOverview
The crystal structure of recombinant rat augmenter of liver regeneration (ALRp) has been determined to 1.8 A. The protein is a homodimer, stabilized by extensive noncovalent interactions and a network of hydrogen bonds, and possesses a noncovalently bound FAD in a motif previously found only in the related protein ERV2p. ALRp functions in vitro as a disulfide oxidase using dithiothreitol as reductant. Reduction of the flavin by DTT occurs under aerobic conditions resulting in a spectrum characteristic of a neutral semiquinone. This semiquinone is stable and is only fully reduced by addition of dithionite. Mutation of either of two cysteine residues that are located adjacent to the FAD results in inactivation of the oxidase activity. A comparison of ALRp with ERV2p is made that reveals a number of significant structural differences, which are related to the in vivo functions of these two proteins. Possible physiological roles of ALR are examined and a hypothesis that it may serve multiple roles is proposed.
About this StructureAbout this Structure
1OQC is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of augmenter of liver regeneration: A mammalian FAD-dependent sulfhydryl oxidase., Wu CK, Dailey TA, Dailey HA, Wang BC, Rose JP, Protein Sci. 2003 May;12(5):1109-18. PMID:12717032
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