RmlC: Difference between revisions

Revision as of 14:04, 17 August 2016

Function

RmlC is an epimerase enzyme of the dTPD-rhamnose pathway. Deoxy-thymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose. L-rhamnose is a sugar component of microbial cell wall and is absent in human^[1].

Relevance

Inhibitors of RmlC can serve as potential antimicrobial drugs^[2].

Structural highlights

The ^[3]. Water molecules shown as red spheres.

3D structures of RmlC3D structures of RmlC

Updated on 17-August-2016

ReferencesReferences

↑ Giraud MF, Leonard GA, Field RA, Berlind C, Naismith JH. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase. Nat Struct Biol. 2000 May;7(5):398-402. PMID:10802738 doi:10.1038/75178
↑ Kantardjieff KA, Kim CY, Naranjo C, Waldo GS, Lekin T, Segelke BW, Zemla A, Park MS, Terwilliger TC, Rupp B. Mycobacterium tuberculosis RmlC epimerase (Rv3465): a promising drug-target structure in the rhamnose pathway. Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):895-902. Epub 2004, Apr 21. PMID:15103135 doi:10.1107/S0907444904005323
↑ Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH. RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787 doi:http://dx.doi.org/10.1016/j.jmb.2006.09.063

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Giraud MF, Leonard GA, Field RA, Berlind C, Naismith JH. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase. Nat Struct Biol. 2000 May;7(5):398-402. PMID:10802738 doi:10.1038/75178

[2] Kantardjieff KA, Kim CY, Naranjo C, Waldo GS, Lekin T, Segelke BW, Zemla A, Park MS, Terwilliger TC, Rupp B. Mycobacterium tuberculosis RmlC epimerase (Rv3465): a promising drug-target structure in the rhamnose pathway. Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):895-902. Epub 2004, Apr 21. PMID:15103135 doi:10.1107/S0907444904005323

[3] Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH. RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787 doi:http://dx.doi.org/10.1016/j.jmb.2006.09.063

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@@ Line 1: / Line 1: @@
-<StructureSection load='2ixc' size='350' side='right' caption='Structure of RmlC complex with dTPD-rhamnose (PDB code [[2ixc]]).' scene='59/597941/Cv/1'>
+<StructureSection load='2ixc' size='450' side='right' caption='Structure of RmlC complex with dTPD-rhamnose (PDB code [[2ixc]]).' scene='59/597941/Cv/1'>
 == Function ==
 '''RmlC''' is an epimerase enzyme of the dTPD-rhamnose pathway.  Deoxy-thymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose.  L-rhamnose is a sugar component of microbial cell wall and is absent in human<ref>PMID:10802738</ref>.
@@ Line 7: / Line 7: @@
 == Structural highlights ==
-The <scene name='59/597941/Cv/3'>active site of RmlC contains the reaction product DTDP-L-rhmnose</scene><ref>PMID:17046787</ref>.
+The <scene name='59/597941/Cv/3'>active site of RmlC contains the reaction product DTDP-L-rhmnose</scene><ref>PMID:17046787</ref>. Water molecules shown as red spheres.
 </StructureSection>