4tq4: Difference between revisions
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==Structure of a UbiA homolog from Archaeoglobus fulgidus bound to DMAPP and Mg2+== | ==Structure of a UbiA homolog from Archaeoglobus fulgidus bound to DMAPP and Mg2+== | ||
<StructureSection load='4tq4' size='340' side='right' caption='[[4tq4]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='4tq4' size='340' side='right' caption='[[4tq4]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMA:DIMETHYLALLYL+DIPHOSPHATE'>DMA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMA:DIMETHYLALLYL+DIPHOSPHATE'>DMA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tq3|4tq3]], [[4tq5|4tq5]], [[4tq6|4tq6]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tq3|4tq3]], [[4tq5|4tq5]], [[4tq6|4tq6]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tq4 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tq4 OCA], [http://pdbe.org/4tq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tq4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4tq4" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 21:39, 11 August 2016
Structure of a UbiA homolog from Archaeoglobus fulgidus bound to DMAPP and Mg2+Structure of a UbiA homolog from Archaeoglobus fulgidus bound to DMAPP and Mg2+
Structural highlights
Publication Abstract from PubMedMembrane-embedded prenyltransferases from the UbiA family catalyze the Mg2+-dependent transfer of a hydrophobic polyprenyl chain onto a variety of acceptor molecules and are involved in the synthesis of molecules that mediate electron transport, including Vitamin K and Coenzyme Q. In humans, missense mutations to the protein UbiA prenyltransferase domain-containing 1 (UBIAD1) are responsible for Schnyder crystalline corneal dystrophy, which is a genetic disease that causes blindness. Mechanistic understanding of this family of enzymes has been hampered by a lack of three-dimensional structures. We have solved structures of a UBIAD1 homolog from Archaeoglobus fulgidus, AfUbiA, in an unliganded form and bound to Mg2+ and two different isoprenyl diphosphates. Functional assays on MenA, a UbiA family member from E. coli, verified the importance of residues involved in Mg2+ and substrate binding. The structural and functional studies led us to propose a mechanism for the prenyl transfer reaction. Disease-causing mutations in UBIAD1 are clustered around the active site in AfUbiA, suggesting the mechanism of catalysis is conserved between the two homologs. Structure of a Membrane-Embedded Prenyltransferase Homologous to UBIAD1.,Huang H, Levin EJ, Liu S, Bai Y, Lockless SW, Zhou M PLoS Biol. 2014 Jul 22;12(7):e1001911. doi: 10.1371/journal.pbio.1001911., eCollection 2014 Jul. PMID:25051182[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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