4wv5: Difference between revisions
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==HEAT SHOCK PROTEIN 70 SUBSTRATE BINDING DOMAIN== | ==HEAT SHOCK PROTEIN 70 SUBSTRATE BINDING DOMAIN== | ||
<StructureSection load='4wv5' size='340' side='right' caption='[[4wv5]], [[Resolution|resolution]] 2.04Å' scene=''> | <StructureSection load='4wv5' size='340' side='right' caption='[[4wv5]], [[Resolution|resolution]] 2.04Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wv7|4wv7]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wv7|4wv7]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wv5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wv5 RCSB], [http://www.ebi.ac.uk/pdbsum/4wv5 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wv5 OCA], [http://pdbe.org/4wv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wv5 RCSB], [http://www.ebi.ac.uk/pdbsum/4wv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wv5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4wv5" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Heat Shock Proteins|Heat Shock Proteins]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:48, 11 August 2016
HEAT SHOCK PROTEIN 70 SUBSTRATE BINDING DOMAINHEAT SHOCK PROTEIN 70 SUBSTRATE BINDING DOMAIN
Structural highlights
Publication Abstract from PubMedThe highly conserved 70 kDa heat shock proteins (Hsp70) play an integral role in proteostasis such that dysregulation has been implicated in numerous diseases. Elucidating the precise role of Hsp70 family members in the cellular context, however, has been hampered by the redundancy and intricate regulation of the chaperone network, and relatively few selective and potent tools. We have characterized a natural product, novolactone, that targets cytosolic and ER-localized isoforms of Hsp70 through a highly conserved covalent interaction at the interface between the substrate-binding and ATPase domains. Biochemical and structural analyses indicate that novolactone disrupts interdomain communication by allosterically inducing a conformational change in the Hsp70 protein to block ATP-induced substrate release and inhibit refolding activities. Thus, novolactone is a valuable tool for exploring the requirements of Hsp70 chaperones in diverse cellular contexts. The Novolactone Natural Product Disrupts the Allosteric Regulation of Hsp70.,Hassan AQ, Kirby CA, Zhou W, Schuhmann T, Kityk R, Kipp DR, Baird J, Chen J, Chen Y, Chung F, Hoepfner D, Movva NR, Pagliarini R, Petersen F, Quinn C, Quinn D, Riedl R, Schmitt EK, Schitter A, Stams T, Studer C, Fortin PD, Mayer MP, Sadlish H Chem Biol. 2014 Dec 23. pii: S1074-5521(14)00415-3. doi:, 10.1016/j.chembiol.2014.11.007. PMID:25544045[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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