4ij4: Difference between revisions
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==Crystal Structure of a Family GH19 chitinase from Bryum coronatum in complex with (GlcNAc)4== | ==Crystal Structure of a Family GH19 chitinase from Bryum coronatum in complex with (GlcNAc)4== | ||
<StructureSection load='4ij4' size='340' side='right' caption='[[4ij4]], [[Resolution|resolution]] 1.58Å' scene=''> | <StructureSection load='4ij4' size='340' side='right' caption='[[4ij4]], [[Resolution|resolution]] 1.58Å' scene=''> | ||
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bcchiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216087 Bryum coronatum])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bcchiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216087 Bryum coronatum])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ij4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ij4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ij4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ij4 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ij4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ij4 OCA], [http://pdbe.org/4ij4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ij4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ij4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ij4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ij4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Chitinase|Chitinase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:20, 11 August 2016
Crystal Structure of a Family GH19 chitinase from Bryum coronatum in complex with (GlcNAc)4Crystal Structure of a Family GH19 chitinase from Bryum coronatum in complex with (GlcNAc)4
Structural highlights
Publication Abstract from PubMedDESCRIPTIONS: The structure of a GH19 chitinase from the moss Bryum coronatum (BcChi-A) in complex with the substrate was examined by X-ray crystallography and NMR spectroscopy in solution. The X-ray crystal structure of the inactive mutant of BcChi-A (BcChi-A-E61A) liganded with chitin tetramer (GlcNAc)4 revealed a clear electron density of the tetramer bound to subsites -2, -1, +1, and +2. Individual sugar residues were recognized by several amino acids at these subsites through a number of hydrogen bonds. This is the first crystal structure of GH19 chitinase liganded with oligosaccharide spanning the catalytic center. NMR titration experiments of chitin oligosaccharides into the BcChi-A-E61A solution showed that the binding mode observed in the crystal structure is similar to that in solution. The C-1 carbon of -1 GlcNAc, the Oepsilon1 atom of the catalytic base (Glu70), and the Ogamma atom of Ser102 form a "triangle" surrounding the catalytic water, and the arrangement structurally validated the proposed catalytic mechanism of GH19 chitinases. The glycosidic linkage between -1 and +1 sugars was found to be twisted and under strain. This situation may contribute to the reduction of activation energy for hydrolysis. The complex structure revealed a more refined mechanism of the chitinase catalysis. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center.,Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T Biochim Biophys Acta. 2014 Feb 25;1844(4):793-802. doi:, 10.1016/j.bbapap.2014.02.013. PMID:24582745[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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