3wl8: Difference between revisions
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==Crystal Structure of pOPH S172A with octanoic acid== | ==Crystal Structure of pOPH S172A with octanoic acid== | ||
<StructureSection load='3wl8' size='340' side='right' caption='[[3wl8]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='3wl8' size='340' side='right' caption='[[3wl8]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wl5|3wl5]], [[3wl6|3wl6]], [[3wl7|3wl7]], [[3wla|3wla]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wl5|3wl5]], [[3wl6|3wl6]], [[3wl7|3wl7]], [[3wla|3wla]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-diketone_hydrolase Beta-diketone hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.7 3.7.1.7] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-diketone_hydrolase Beta-diketone hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.7 3.7.1.7] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wl8 RCSB], [http://www.ebi.ac.uk/pdbsum/3wl8 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wl8 OCA], [http://pdbe.org/3wl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wl8 RCSB], [http://www.ebi.ac.uk/pdbsum/3wl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wl8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3wl8" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:17, 11 August 2016
Crystal Structure of pOPH S172A with octanoic acidCrystal Structure of pOPH S172A with octanoic acid
Structural highlights
Publication Abstract from PubMedThe ever-increasing production and use of polyvinyl alcohol (PVA) threaten our environment. Yet PVA can be assimilated by microbes in two steps: oxidation and cleavage. Here we report novel alpha/beta-hydrolase structures of oxidized PVA hydrolase (OPH) from two known PVA-degrading organisms, Sphingopyxis sp. 113P3 and Pseudomonas sp. VM15C, including complexes with substrate analogues, acetylacetone and caprylate. The active site is covered by a lid-like beta-ribbon. Unlike other esterase and amidase, OPH is unique in cleaving the CC bond of beta-diketone, although it has a catalytic triad similar to that of most alpha/beta-hydrolases. Analysis of the crystal structures suggests a double-oxyanion-hole mechanism, previously only found in thiolase cleaving beta-ketoacyl-CoA. Three mutations in the lid region showed enhanced activity, with potential in industrial applications. Structural insights into enzymatic degradation of oxidized polyvinyl alcohol.,Yang Y, Ko TP, Liu L, Li J, Huang CH, Chan HC, Ren F, Jia D, Wang AH, Guo RT, Chen J, Du G Chembiochem. 2014 Sep 5;15(13):1882-6. doi: 10.1002/cbic.201402166. Epub 2014 Jul, 8. PMID:25044912[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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