4mvq: Difference between revisions
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==Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel== | ==Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel== | ||
<StructureSection load='4mvq' size='340' side='right' caption='[[4mvq]], [[Resolution|resolution]] 3.40Å' scene=''> | <StructureSection load='4mvq' size='340' side='right' caption='[[4mvq]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ms2|4ms2]], [[4mtf|4mtf]], [[4mtg|4mtg]], [[4mto|4mto]], [[4mvm|4mvm]], [[4mvo|4mvo]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ms2|4ms2]], [[4mtf|4mtf]], [[4mtg|4mtg]], [[4mto|4mto]], [[4mvm|4mvm]], [[4mvo|4mvo]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Abu_1752 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=367737 ARCB4])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Abu_1752 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=367737 ARCB4])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mvq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mvq RCSB], [http://www.ebi.ac.uk/pdbsum/4mvq PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mvq OCA], [http://pdbe.org/4mvq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mvq RCSB], [http://www.ebi.ac.uk/pdbsum/4mvq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mvq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4mvq" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 19:44, 11 August 2016
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium ChannelStructural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Structural highlights
Publication Abstract from PubMedVoltage-gated calcium (CaV) channels catalyse rapid, highly selective influx of Ca2+ into cells despite a 70-fold higher extracellular concentration of Na+. How CaV channels solve this fundamental biophysical problem remains unclear. Here we report physiological and crystallographic analyses of a calcium selectivity filter constructed in the homotetrameric bacterial NaV channel NaVAb. Our results reveal interactions of hydrated Ca2+ with two high-affinity Ca2+-binding sites followed by a third lower-affinity site that would coordinate Ca2+ as it moves inward. At the selectivity filter entry, Site 1 is formed by four carboxyl side chains, which have a critical role in determining Ca2+ selectivity. Four carboxyls plus four backbone carbonyls form Site 2, which is targeted by the blocking cations Cd2+ and Mn2+, with single occupancy. The lower-affinity Site 3 is formed by four backbone carbonyls alone, which mediate exit into the central cavity. This pore architecture suggests a conduction pathway involving transitions between two main states with one or two hydrated Ca2+ ions bound in the selectivity filter and supports a 'knock-off' mechanism of ion permeation through a stepwise-binding process. The multi-ion selectivity filter of our CaVAb model establishes a structural framework for understanding the mechanisms of ion selectivity and conductance by vertebrate CaV channels. Structural basis for Ca selectivity of a voltage-gated calcium channel.,Tang L, Gamal El-Din TM, Payandeh J, Martinez GQ, Heard TM, Scheuer T, Zheng N, Catterall WA Nature. 2013 Nov 24. doi: 10.1038/nature12775. PMID:24270805[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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