4e08: Difference between revisions
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==Crystal structure of Drosophila melanogaster DJ-1beta== | ==Crystal structure of Drosophila melanogaster DJ-1beta== | ||
<StructureSection load='4e08' size='340' side='right' caption='[[4e08]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='4e08' size='340' side='right' caption='[[4e08]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4e08]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4e08]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E08 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E08 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG1349, dj-1 beta, dj-1-beta, dj-1beta, Dmel_CG1349 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG1349, dj-1 beta, dj-1-beta, dj-1beta, Dmel_CG1349 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e08 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e08 RCSB], [http://www.ebi.ac.uk/pdbsum/4e08 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e08 OCA], [http://pdbe.org/4e08 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e08 RCSB], [http://www.ebi.ac.uk/pdbsum/4e08 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e08 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4e08" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Drome]] | ||
[[Category: Lin, J]] | [[Category: Lin, J]] | ||
[[Category: Prahlad, J]] | [[Category: Prahlad, J]] | ||
Revision as of 19:10, 11 August 2016
Crystal structure of Drosophila melanogaster DJ-1betaCrystal structure of Drosophila melanogaster DJ-1beta
Structural highlights
Publication Abstract from PubMedDJ-1 is a conserved, disease-associated protein that protects against oxidative stress and mitochondrial damage in multiple organisms. Human DJ-1 contains a functionally essential cysteine residue (Cys106) whose oxidation is important for regulating protein function by an unknown mechanism. This residue is well-conserved in other DJ-1 homologues, including two (DJ-1alpha and DJ-1beta) in Drosophila melanogaster. Because D. melanogaster is a powerful model system for studying DJ-1 function, we have determined the crystal structure and impact of cysteine oxidation on Drosophila DJ-1beta. The structure of D. melanogaster DJ-1beta is similar to that of human DJ-1, although two important residues in the human protein, Met26 and His126, are not conserved in DJ-1beta. His126 in human DJ-1 is substituted with a tyrosine in DJ-1beta, and this residue is not able to compose a putative catalytic dyad with Cys106 that was proposed to be important in the human protein. The reactive cysteine in DJ-1 is oxidized readily to the cysteine-sulfinic acid in both flies and humans, and this may regulate the cytoprotective function of the protein. We show that the oxidation of this conserved cysteine residue to its sulfinate form (Cys-SO(2)(-)) results in considerable thermal stabilization of both Drosophila DJ-1beta and human DJ-1. Therefore, protein stabilization is one potential mechanism by which cysteine oxidation may regulate DJ-1 function in vivo. More generally, most close DJ-1 homologues are likely stabilized by cysteine-sulfinic acid formation but destabilized by further oxidation, suggesting that they are biphasically regulated by oxidative modification. Conservation of Oxidative Protein Stabilization in an Insect Homologue of Parkinsonism-Associated Protein DJ-1.,Lin J, Prahlad J, Wilson MA Biochemistry. 2012 Apr 24. PMID:22515803[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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