4lvz: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of the THF riboswitch bound to 2,6-diaminopurine== | ==Structure of the THF riboswitch bound to 2,6-diaminopurine== | ||
<StructureSection load='4lvz' size='340' side='right' caption='[[4lvz]], [[Resolution|resolution]] 1.77Å' scene=''> | <StructureSection load='4lvz' size='340' side='right' caption='[[4lvz]], [[Resolution|resolution]] 1.77Å' scene=''> | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6AP:9H-PURINE-2,6-DIAMINE'>6AP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6AP:9H-PURINE-2,6-DIAMINE'>6AP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lw0|4lw0]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lw0|4lw0]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lvz RCSB], [http://www.ebi.ac.uk/pdbsum/4lvz PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lvz OCA], [http://pdbe.org/4lvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lvz RCSB], [http://www.ebi.ac.uk/pdbsum/4lvz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lvz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 15: | Line 16: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4lvz" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Riboswitch|Riboswitch]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 18:21, 11 August 2016
Structure of the THF riboswitch bound to 2,6-diaminopurineStructure of the THF riboswitch bound to 2,6-diaminopurine
Structural highlights
Publication Abstract from PubMedThe tetrahydrofolate (THF) riboswitch regulates folate transport and metabolism in a number of Firmicutes by cooperatively binding two molecules of THF. To further understand this riboswitch's specificity for THF, binding and regulatory activity of a series of THF analogs and antifolates were examined. Our data reveal that although binding is dominated by the RNA's interactions with the pterin moiety, the para-aminobenzoic acid (pABA) moiety plays a significant role in transcriptional regulation. Further, we find that adenine and several other analogs bind with high affinity by an alternative binding mechanism. Despite a similar affinity to THF, adenine is a poor regulator of transcriptional attenuation. These results demonstrate that binding alone does not determine a compound's effectiveness in regulating the activity of the riboswitch-a complication in current efforts to develop antimicrobials that target these RNAs. A Disconnect between High-Affinity Binding and Efficient Regulation by Antifolates and Purines in the Tetrahydrofolate Riboswitch.,Trausch JJ, Batey RT Chem Biol. 2014 Feb 20;21(2):205-16. doi: 10.1016/j.chembiol.2013.11.012. Epub, 2014 Jan 2. PMID:24388757[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Batey, R T
- Trausch, J J
- 6-diaminopurine
- Aptamer
- Bacillus subtili
- Bacterial
- Bacterial protein
- Base sequence
- Binding site
- Calorimetry
- Folic acid
- Gene expression regulation
- Genetic
- Guanine
- Leucovorin
- Ligand
- Magnesium
- Molecular sequence data
- Mrna
- Ncrna
- Nucleic acid conformation
- Nucleotide
- Point mutation
- Protein binding
- Protein structure
- Pseudoknot
- Regulation
- Riboswitch
- Rna
- S-adenosylmethionine
- Secondary
- Streptococcus mutan
- Terminator region
- Tetrahydrofolate
- Thermodynamic
- Three-way junction
- Transcription