1nlo: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 1nlo |SIZE=350|CAPTION= <scene name='initialview01'>1nlo</scene> | |PDB= 1nlo |SIZE=350|CAPTION= <scene name='initialview01'>1nlo</scene> | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=MN1:4-CARBOXYPIPERIDINE'>MN1</scene>, <scene name='pdbligand=MN2:1-CARBOXYETHYLAMINOMETHYL-4-AMINOMETHYLBENZENE'>MN2</scene>, <scene name='pdbligand=MN7:1-CARBOXY-4-ISOBUTYLAMINOMETHYLBENZENE'>MN7</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] </span> | ||
|GENE= CHICKEN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | |GENE= CHICKEN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nlo OCA], [http://www.ebi.ac.uk/pdbsum/1nlo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nlo RCSB]</span> | |||
}} | }} | ||
| Line 28: | Line 31: | ||
[[Category: Schreiber, S L.]] | [[Category: Schreiber, S L.]] | ||
[[Category: Shirai, F.]] | [[Category: Shirai, F.]] | ||
[[Category: complex (transferase/peptide)]] | [[Category: complex (transferase/peptide)]] | ||
[[Category: ligand]] | [[Category: ligand]] | ||
| Line 36: | Line 37: | ||
[[Category: src]] | [[Category: src]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:32:09 2008'' | ||
Revision as of 22:32, 30 March 2008
| |||||||
| Ligands: | , , , , | ||||||
| Gene: | CHICKEN (Gallus gallus) | ||||||
| Activity: | Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF SIGNAL TRANSDUCTION PROTEIN, NMR, MINIMIZED AVERAGE STRUCTURE
OverviewOverview
BACKGROUND: Protein-structure-based combinatorial chemistry has recently been used to discover several ligands containing non-peptide binding elements to the Src SH3 domain. The encoded library used has the form Cap-M1-M2-M3-PLPPLP, in which the Cap and Mi's are composed of a diverse set of organic monomers. The PLPPLP portion provided a structural bias directing the non-peptide fragment Cap-M1-M2-M3 to the SH3 specificity pocket. Fifteen ligands were selected from > 1.1 million distinct compounds. The structural basis for selection was unknown. RESULTS: The solution structures of the Src SH3 domain complexed with two ligands containing non-peptide elements selected from the library were determined by multidimensional NMR spectroscopy. The non-peptide moieties of the ligands interact with the specificity pocket of Src SH3 domain differently from peptides complexed with SH3 domains. Structural information about the ligands was used to design various homologs, whose affinities for the SH3 domain were measured. The results provide a structural basis for understanding the selection of a few optimal ligands from a large library. CONCLUSIONS: The cycle of protein-structure-based combinatorial chemistry followed by structure determination of the few highest affinity ligands provides a powerful new tool for the field of molecular recognition.
About this StructureAbout this Structure
1NLO is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
ReferenceReference
Molecular basis for the binding of SH3 ligands with non-peptide elements identified by combinatorial synthesis., Feng S, Kapoor TM, Shirai F, Combs AP, Schreiber SL, Chem Biol. 1996 Aug;3(8):661-70. PMID:8807900
Page seeded by OCA on Sun Mar 30 22:32:09 2008