4aoe: Difference between revisions
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==Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)== | ==Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)== | ||
<StructureSection load='4aoe' size='340' side='right' caption='[[4aoe]], [[Resolution|resolution]] 5.80Å' scene=''> | <StructureSection load='4aoe' size='340' side='right' caption='[[4aoe]], [[Resolution|resolution]] 5.80Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4aoe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Biomphalaria_glabrata Biomphalaria glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AOE FirstGlance]. <br> | <table><tr><td colspan='2'>[[4aoe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Biomphalaria_glabrata Biomphalaria glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AOE FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aod|4aod]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aod|4aod]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aoe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aoe RCSB], [http://www.ebi.ac.uk/pdbsum/4aoe PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aoe OCA], [http://pdbe.org/4aoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aoe RCSB], [http://www.ebi.ac.uk/pdbsum/4aoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aoe ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4aoe" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
Revision as of 13:41, 11 August 2016
Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)
Structural highlights
Publication Abstract from PubMedNicotinic acetylcholine receptors (nAChR) play important neurophysiological roles and are of considerable medical relevance. They have been studied extensively, greatly facilitated by the gastropod acetylcholine-binding proteins (AChBP) which represent soluble structural and functional homologues of the ligand-binding domain of nAChR. All these proteins are ring-like pentamers. Here we report that AChBP exists in the hemolymph of the planorbid snail Biomphalaria glabrata (vector of the schistosomiasis parasite) as a regular pentagonal dodecahedron, 22 nm in diameter (12 pentamers, 60 active sites). We sequenced and recombinantly expressed two approximately 25 kDa polypeptides (BgAChBP1 and BgAChBP2) with a specific active site, N-glycan site and disulfide bridge variation. We also provide the exon/intron structures. Recombinant BgAChBP1 formed pentamers and dodecahedra, recombinant BgAChBP2 formed pentamers and probably disulfide-bridged di-pentamers, but not dodecahedra. Three-dimensional electron cryo-microscopy (3D-EM) yielded a 3D reconstruction of the dodecahedron with a resolution of 6 A. Homology models of the pentamers docked to the 6 A structure revealed opportunities for chemical bonding at the inter-pentamer interfaces. Definition of the ligand-binding pocket and the gating C-loop in the 6 A structure suggests that 3D-EM might lead to the identification of functional states in the BgAChBP dodecahedron. Acetylcholine-Binding Protein in the Hemolymph of the Planorbid Snail Biomphalaria glabrata Is a Pentagonal Dodecahedron (60 Subunits).,Saur M, Moeller V, Kapetanopoulos K, Braukmann S, Gebauer W, Tenzer S, Markl J PLoS One. 2012;7(8):e43685. Epub 2012 Aug 20. PMID:22916297[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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