2v6e: Difference between revisions
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==PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA== | ==PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA== | ||
<StructureSection load='2v6e' size='340' side='right' caption='[[2v6e]], [[Resolution|resolution]] 3.20Å' scene=''> | <StructureSection load='2v6e' size='340' side='right' caption='[[2v6e]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2v6e]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2v6e]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phiko2 Bacteriophage phiko2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V6E FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB], [http://www.ebi.ac.uk/pdbsum/2v6e PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [http://pdbe.org/2v6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB], [http://www.ebi.ac.uk/pdbsum/2v6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v6e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2v6e" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Bacteriophage phiko2]] | ||
[[Category: Aihara, H]] | [[Category: Aihara, H]] | ||
[[Category: Ellenberger, T]] | [[Category: Ellenberger, T]] | ||
Revision as of 13:33, 11 August 2016
PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNAPROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA
Structural highlights
Publication Abstract from PubMedThe termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate. An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres.,Aihara H, Huang WM, Ellenberger T Mol Cell. 2007 Sep 21;27(6):901-13. PMID:17889664[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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