1nk0: Difference between revisions
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|PDB= 1nk0 |SIZE=350|CAPTION= <scene name='initialview01'>1nk0</scene>, resolution 1.70Å | |PDB= 1nk0 |SIZE=350|CAPTION= <scene name='initialview01'>1nk0</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1njw|1NJW]], [[1njx|1NJX]], [[1njy|1NJY]], [[1njz|1NJZ]], [[1nk4|1NK4]], [[1nk5|1NK5]], [[1nk6|1NK6]], [[1nk7|1NK7]], [[1nk8|1NK8]], [[1nk9|1NK9]], [[1nkb|1NKB]], [[1nkc|1NKC]], [[1nke|1NKE]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nk0 OCA], [http://www.ebi.ac.uk/pdbsum/1nk0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nk0 RCSB]</span> | |||
}} | }} | ||
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[[Category: Beese, L S.]] | [[Category: Beese, L S.]] | ||
[[Category: Johnson, S J.]] | [[Category: Johnson, S J.]] | ||
[[Category: dna mismatch]] | [[Category: dna mismatch]] | ||
[[Category: dna polymerase i]] | [[Category: dna polymerase i]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:27 2008'' | ||
Revision as of 22:31, 30 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Related: | 1NJW, 1NJX, 1NJY, 1NJZ, 1NK4, 1NK5, 1NK6, 1NK7, 1NK8, 1NK9, 1NKB, 1NKC, 1NKE
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE
OverviewOverview
Accurate DNA replication is essential for genomic stability. One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a "short-term memory" of replication errors, responding to mismatches up to four base pairs in from the primer terminus. Here we a present a structural characterization of all 12 possible mismatches captured at the growing primer terminus in the active site of a polymerase. Our observations suggest four mechanisms that lead to mismatch-induced stalling of the polymerase. Furthermore, we have observed the effects of extending a mismatch up to six base pairs from the primer terminus and find that long-range distortions in the DNA transmit the presence of the mismatch back to the enzyme active site, suggesting the structural basis for the short-term memory of replication errors.
About this StructureAbout this Structure
1NK0 is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:15035983
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