4mi7: Difference between revisions
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==Crystal Structure of Salmonella Effector Protein GtgE== | ==Crystal Structure of Salmonella Effector Protein GtgE== | ||
<StructureSection load='4mi7' size='340' side='right' caption='[[4mi7]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='4mi7' size='340' side='right' caption='[[4mi7]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gtgE, SL1344_0995 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216597 SALTS])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gtgE, SL1344_0995 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216597 SALTS])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/4mi7 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [http://pdbe.org/4mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/4mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mi7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4mi7" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 10:59, 11 August 2016
Crystal Structure of Salmonella Effector Protein GtgECrystal Structure of Salmonella Effector Protein GtgE
Structural highlights
Publication Abstract from PubMedGtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 A resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections. Structural and enzymatic characterization of a host-specificity determinant from Salmonella.,Kohler AC, Spano S, Galan JE, Stebbins CE Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):384-91. doi:, 10.1107/S1399004713028393. Epub 2014 Jan 29. PMID:24531472[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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