1n67: Difference between revisions
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|PDB= 1n67 |SIZE=350|CAPTION= <scene name='initialview01'>1n67</scene>, resolution 1.90Å | |PDB= 1n67 |SIZE=350|CAPTION= <scene name='initialview01'>1n67</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1amx|1AMX]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n67 OCA], [http://www.ebi.ac.uk/pdbsum/1n67 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n67 RCSB]</span> | |||
}} | }} | ||
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[[Category: Rajashankar, K R.]] | [[Category: Rajashankar, K R.]] | ||
[[Category: Wann, E R.]] | [[Category: Wann, E R.]] | ||
[[Category: clumping factor]] | [[Category: clumping factor]] | ||
[[Category: dev-igg]] | [[Category: dev-igg]] | ||
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[[Category: staphylococcus aureus]] | [[Category: staphylococcus aureus]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:00 2008'' | ||
Revision as of 22:26, 30 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Related: | 1AMX
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Clumping Factor A from Staphylococcus aureus
OverviewOverview
We report here the crystal structure of the minimal ligand-binding segment of the Staphylococcus aureus MSCRAMM, clumping factor A. This fibrinogen-binding segment contains two similarly folded domains. The fold observed is a new variant of the immunoglobulin motif that we have called DE-variant or the DEv-IgG fold. This subgroup includes the ligand-binding domain of the collagen-binding S.aureus MSCRAMM CNA, and many other structures previously classified as jelly rolls. Structure predictions suggest that the four fibrinogen-binding S.aureus MSCRAMMs identified so far would also contain the same DEv-IgG fold. A systematic docking search using the C-terminal region of the fibrinogen gamma-chain as a probe suggested that a hydrophobic pocket formed between the two DEv-IgG domains of the clumping factor as the ligand-binding site. Mutagenic substitution of residues Tyr256, Pro336, Tyr338 and Lys389 in the clumping factor, which are proposed to contact the terminal residues (408)AGDV(411) of the gamma-chain, resulted in proteins with no or markedly reduced affinity for fibrinogen.
About this StructureAbout this Structure
1N67 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A., Deivanayagam CC, Wann ER, Chen W, Carson M, Rajashankar KR, Hook M, Narayana SV, EMBO J. 2002 Dec 16;21(24):6660-72. PMID:12485987
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