3win: Difference between revisions
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==Clostridium botulinum Hemagglutinin== | ==Clostridium botulinum Hemagglutinin== | ||
<StructureSection load='3win' size='340' side='right' caption='[[3win]], [[Resolution|resolution]] 3.50Å' scene=''> | <StructureSection load='3win' size='340' side='right' caption='[[3win]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
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<table><tr><td colspan='2'>[[3win]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_b Clostridium botulinum b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WIN FirstGlance]. <br> | <table><tr><td colspan='2'>[[3win]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_b Clostridium botulinum b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WIN FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ha3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B]), hem17/B, ha2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B]), ha1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ha3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B]), hem17/B, ha2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B]), ha1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3win FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3win OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3win RCSB], [http://www.ebi.ac.uk/pdbsum/3win PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3win FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3win OCA], [http://pdbe.org/3win PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3win RCSB], [http://www.ebi.ac.uk/pdbsum/3win PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3win ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3win" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 23:51, 5 August 2016
Clostridium botulinum HemagglutininClostridium botulinum Hemagglutinin
Structural highlights
Publication Abstract from PubMedClostridium botulinum HA is a component of the large botulinum neurotoxin complex and is critical for its oral toxicity. HA plays multiple roles in toxin penetration in the gastrointestinal tract, including protection from the digestive environment, binding to the intestinal mucosal surface, and disruption of the epithelial barrier. At least two properties of HA contribute to these roles: the sugar-binding activity and the barrier-disrupting activity that depends on E-cadherin binding of HA. HA consists of three different proteins, HA1, HA2, and HA3, whose structures have been partially solved and are made up mainly of beta-strands. Here, we demonstrate structural and functional reconstitution of whole HA and present the complete structure of HA of serotype B determined by x-ray crystallography at 3.5 A resolution. This structure reveals whole HA to be a huge triskelion-shaped molecule. Our results suggest that whole HA is functionally and structurally separable into two parts: HA1, involved in recognition of cell-surface carbohydrates, and HA2-HA3, involved in paracellular barrier disruption by E-cadherin binding. Crystal Structure of Clostridium botulinum Whole Hemagglutinin Reveals a Huge Triskelion-shaped Molecular Complex.,Amatsu S, Sugawara Y, Matsumura T, Kitadokoro K, Fujinaga Y J Biol Chem. 2013 Dec 6;288(49):35617-25. doi: 10.1074/jbc.M113.521179. Epub 2013, Oct 28. PMID:24165130[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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