4kw3: Difference between revisions
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==Crystal structure of the non-structural protein 1 N-terminal origin-recognition/nickase domain from the emerging human bocavirus== | ==Crystal structure of the non-structural protein 1 N-terminal origin-recognition/nickase domain from the emerging human bocavirus== | ||
<StructureSection load='4kw3' size='340' side='right' caption='[[4kw3]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='4kw3' size='340' side='right' caption='[[4kw3]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4kw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_bocavirus Human bocavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KW3 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4kw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_bocavirus Human bocavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KW3 FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=329641 Human bocavirus])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=329641 Human bocavirus])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kw3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kw3 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kw3 OCA], [http://pdbe.org/4kw3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kw3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kw3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kw3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4kw3" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 23:23, 5 August 2016
Crystal structure of the non-structural protein 1 N-terminal origin-recognition/nickase domain from the emerging human bocavirusCrystal structure of the non-structural protein 1 N-terminal origin-recognition/nickase domain from the emerging human bocavirus
Structural highlights
Publication Abstract from PubMedHuman bocavirus is a newly identified, globally prevalent, parvovirus that is associated with respiratory infection in infant and young children. Parvoviruses encode a large non-structural protein 1 (NS1) that is essential for replication of the viral single-stranded DNA genome and DNA packaging and may play versatile roles in virus:host interaction. Here we report the structure of the human parvovirus NS1 N-terminal domain, the first for any autonomous parvovirus. The structure shows an overall fold that is canonical to the histidine-hydrophobic-histidine superfamily of nucleases, which integrates two distinct DNA-binding sites: a positively charged region mediated by a surface hairpin (residues 190-198) that is responsible for recognition of the viral origin of replication of the double-stranded DNA nature; and the nickase active site that binds to the single-stranded DNA substrate for site-specific cleavage. The structure reveals an acidic-residue-rich sub-domain that is present in bocavirus NS1 proteins but not in the NS1 orthologs in erythrovirus or dependovirus, which may mediate bocavirus-specific interaction with DNA or potential host factors. These results provide insights into recognition of the origin of replication and nicking of DNA during bocavirus genome replication. Mapping of variable amino acid residues of NS1s from four HBoV species onto the structure shows a scattered pattern, but the origin-recognition site and the nuclease active site are invariable, suggesting potential targets for antivirals against this clade of highly diverse human viruses. Structure of the NS1 protein N-terminal origin-recognition/nickase domain from the emerging human bocavirus.,Tewary SK, Zhao H, Shen W, Qiu J, Tang L J Virol. 2013 Aug 21. PMID:23966383[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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