2ylc: Difference between revisions
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==STRUCTURE OF SALMONELLA TYPHIMURIUM HFQ IN COMPLEX WITH U6 RNA== | ==STRUCTURE OF SALMONELLA TYPHIMURIUM HFQ IN COMPLEX WITH U6 RNA== | ||
<StructureSection load='2ylc' size='340' side='right' caption='[[2ylc]], [[Resolution|resolution]] 1.30Å' scene=''> | <StructureSection load='2ylc' size='340' side='right' caption='[[2ylc]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ylc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2ylc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YLC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ylb|2ylb]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ylb|2ylb]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ylc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ylc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ylc RCSB], [http://www.ebi.ac.uk/pdbsum/2ylc PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ylc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ylc OCA], [http://pdbe.org/2ylc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ylc RCSB], [http://www.ebi.ac.uk/pdbsum/2ylc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ylc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/HFQ_SALTY HFQ_SALTY]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity). Plays a central regulatory role in the microbial response to space flight conditions. Is essential for virulence and is required for efficient invasion of non-phagocytic cells.[HAMAP-Rule:MF_00436]<ref>PMID:17163975</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2ylc" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Salty]] | ||
[[Category: Sauer, E]] | [[Category: Sauer, E]] | ||
[[Category: Weichenrieder, O]] | [[Category: Weichenrieder, O]] | ||
Revision as of 21:37, 5 August 2016
STRUCTURE OF SALMONELLA TYPHIMURIUM HFQ IN COMPLEX WITH U6 RNASTRUCTURE OF SALMONELLA TYPHIMURIUM HFQ IN COMPLEX WITH U6 RNA
Structural highlights
Function[HFQ_SALTY] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity). Plays a central regulatory role in the microbial response to space flight conditions. Is essential for virulence and is required for efficient invasion of non-phagocytic cells.[HAMAP-Rule:MF_00436][1] Publication Abstract from PubMedThe homohexameric (L)Sm protein Hfq is a central mediator of small RNA-based gene regulation in bacteria. Hfq recognizes small regulatory RNAs (sRNAs) specifically, despite their structural diversity. This specificity could not be explained by previously described RNA-binding modes of Hfq. Here we present a distinct and preferred mode of Hfq-RNA interaction that involves the direct recognition of a uridine-rich RNA 3' end. This feature is common in bacterial RNA transcripts as a consequence of Rho-independent transcription termination and hence likely contributes significantly to the general recognition of sRNAs by Hfq. Isothermal titration calorimetry shows nanomolar affinity between Salmonella typhimurium Hfq and a hexauridine substrate. We determined a crystal structure of the complex that reveals a constricted RNA backbone conformation in the proximal RNA-binding site of Hfq, allowing for a direct protein contact of the 3' hydroxyl group. A free 3' hydroxyl group is crucial for the high-affinity interaction with Hfq also in the context of a full-length sRNA substrate, RybB. The capacity of Hfq to occupy and sequester the RNA 3' end has important implications for the mechanisms by which Hfq is thought to affect sRNA stability, turnover, and regulation. Structural basis for RNA 3'-end recognition by Hfq.,Sauer E, Weichenrieder O Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13065-70. Epub 2011 Jul 7. PMID:21737752[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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