4mgm: Difference between revisions
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==Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly== | ==Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly== | ||
<StructureSection load='4mgm' size='340' side='right' caption='[[4mgm]], [[Resolution|resolution]] 3.20Å' scene=''> | <StructureSection load='4mgm' size='340' side='right' caption='[[4mgm]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mgn|4mgn]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mgn|4mgn]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mgm RCSB], [http://www.ebi.ac.uk/pdbsum/4mgm PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgm OCA], [http://pdbe.org/4mgm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mgm RCSB], [http://www.ebi.ac.uk/pdbsum/4mgm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mgm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4mgm" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[TRNA|TRNA]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 21:11, 5 August 2016
Crystal structure of the in vitro transcribed G. kaustophilus tRNA-GlyCrystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly
Structural highlights
Publication Abstract from PubMedT box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 A resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules. Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.,Grigg JC, Ke A Structure. 2013 Oct 1. pii: S0969-2126(13)00346-8. doi:, 10.1016/j.str.2013.09.001. PMID:24095061[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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