4ekb: Difference between revisions
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==Initial Thaumatin Structure for Radiation Damage Experiment at 100 K== | ==Initial Thaumatin Structure for Radiation Damage Experiment at 100 K== | ||
<StructureSection load='4ekb' size='340' side='right' caption='[[4ekb]], [[Resolution|resolution]] 1.52Å' scene=''> | <StructureSection load='4ekb' size='340' side='right' caption='[[4ekb]], [[Resolution|resolution]] 1.52Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ek0|4ek0]], [[4eka|4eka]], [[4ekh|4ekh]], [[4eko|4eko]], [[4ekt|4ekt]], [[4el2|4el2]], [[4el3|4el3]], [[4el7|4el7]], [[4ela|4ela]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ek0|4ek0]], [[4eka|4eka]], [[4ekh|4ekh]], [[4eko|4eko]], [[4ekt|4ekt]], [[4el2|4el2]], [[4el3|4el3]], [[4el7|4el7]], [[4ela|4ela]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ekb RCSB], [http://www.ebi.ac.uk/pdbsum/4ekb PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekb OCA], [http://pdbe.org/4ekb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ekb RCSB], [http://www.ebi.ac.uk/pdbsum/4ekb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ekb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ekb" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:29, 5 August 2016
Initial Thaumatin Structure for Radiation Damage Experiment at 100 KInitial Thaumatin Structure for Radiation Damage Experiment at 100 K
Structural highlights
Function[THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis. Publication Abstract from PubMedThe spatial distribution of radiation damage (assayed by increases in atomic B factors) to thaumatin and urease crystals at temperatures ranging from 25 to 300 K is reported. The nature of the damage changes dramatically at approximately 180 K. Above this temperature the role of solvent diffusion is apparent in thaumatin crystals, as solvent-exposed turns and loops are especially sensitive. In urease, a flap covering the active site is the most sensitive part of the molecule and nearby loops show enhanced sensitivity. Below 180 K sensitivity is correlated with poor local packing, especially in thaumatin. At all temperatures, the component of the damage that is spatially uniform within the unit cell accounts for more than half of the total increase in the atomic B factors and correlates with changes in mosaicity. This component may arise from lattice-level, rather than local, disorder. The effects of primary structure on radiation sensitivity are small compared with those of tertiary structure, local packing, solvent accessibility and crystal contacts. Spatial distribution of radiation damage to crystalline proteins at 25-300 K.,Warkentin M, Badeau R, Hopkins JB, Thorne RE Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1108-17. doi:, 10.1107/S0907444912021361. Epub 2012 Aug 18. PMID:22948911[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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