3qbg: Difference between revisions
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==Anion-free blue form of pharaonis halorhodopsin== | ==Anion-free blue form of pharaonis halorhodopsin== | ||
<StructureSection load='3qbg' size='340' side='right' caption='[[3qbg]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3qbg' size='340' side='right' caption='[[3qbg]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=22B:BACTERIORUBERIN'>22B</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=22B:BACTERIORUBERIN'>22B</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a7k|3a7k]], [[3qbi|3qbi]], [[3qbk|3qbk]], [[3qbl|3qbl]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a7k|3a7k]], [[3qbi|3qbi]], [[3qbk|3qbk]], [[3qbl|3qbl]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qbg RCSB], [http://www.ebi.ac.uk/pdbsum/3qbg PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qbg OCA], [http://pdbe.org/3qbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qbg RCSB], [http://www.ebi.ac.uk/pdbsum/3qbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qbg ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3qbg" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
Revision as of 18:21, 5 August 2016
Anion-free blue form of pharaonis halorhodopsinAnion-free blue form of pharaonis halorhodopsin
Structural highlights
Publication Abstract from PubMedHalorhodopsin from Natronomonas pharaonis (pHR) was previously crystallized into a monoclinic space group C2, and the structure of the chloride-bound purple form was determined. Here, we report the crystal structures of two chloride-free forms of pHR, that is, an O-like blue form and an M-like yellow form. When the C2 crystal was soaked in a chloride-free alkaline solution, the protein packing was largely altered and the yellow form containing all-trans retinal was generated. Upon neutralization, this yellow form was converted into the blue form. From structural comparison of the different forms of pHR, it was shown that the removal of a chloride ion from the primary binding site (site I), which is located between the retinal Schiff base and Thr126, is accompanied by such a deformation of helix C that the side chain of Thr126 moves toward helix G, leading to a significant shrinkage of site I. A large structural change is also induced in the chloride uptake pathway, where a flip motion of the side chain of Glu234 is accompanied by large movements of the surrounding aromatic residues. Irrespective of different charge distributions at the active site, there was no large difference in the structures of the yellow form and the blue form. It is shown that the yellow-to-purple transition is initiated by the entrance of one water and one HCl to the active site, where the proton and the chloride ion in HCl are transferred to the Schiff base and site I, respectively. Crystal structures of an O-like blue form and an anion-free yellow form of pharaonis halorhodopsin.,Kanada S, Takeguchi Y, Murakami M, Ihara K, Kouyama T J Mol Biol. 2011 Oct 14;413(1):162-76. Epub 2011 Aug 16. PMID:21871461[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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