1mi7: Difference between revisions
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|PDB= 1mi7 |SIZE=350|CAPTION= <scene name='initialview01'>1mi7</scene>, resolution 2.50Å | |PDB= 1mi7 |SIZE=350|CAPTION= <scene name='initialview01'>1mi7</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene> | |LIGAND= <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= trpR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= trpR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1wrp|1WRP]], [[2wrp|2WRP]], [[3wrp|3WRP]], [[1tro|1TRO]], [[1trr|1TRR]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [http://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB]</span> | |||
}} | }} | ||
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[[Category: Carey, J.]] | [[Category: Carey, J.]] | ||
[[Category: Lawson, C L.]] | [[Category: Lawson, C L.]] | ||
[[Category: alcohol induced conformational rearrangement]] | [[Category: alcohol induced conformational rearrangement]] | ||
[[Category: dna binding protein]] | [[Category: dna binding protein]] | ||
[[Category: domain swapping]] | [[Category: domain swapping]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:21 2008'' | ||
Revision as of 22:16, 30 March 2008
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| , resolution 2.50Å | |||||||
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| Ligands: | |||||||
| Gene: | trpR (Escherichia coli) | ||||||
| Related: | 1WRP, 2WRP, 3WRP, 1TRO, 1TRR
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol
OverviewOverview
The E. coli trp repressor (trpR) homodimer recognizes its palindromic DNA binding site through a pair of flexible helix-turn-helix (HTH) motifs displayed on an intertwined helical core. Flexible N-terminal arms mediate association between dimers bound to tandem DNA sites. The 2.5 A X-ray structure of trpR crystallized in 30% (v/v) isopropanol reveals a substantial conformational rearrangement of HTH motifs and N-terminal arms, with the protein appearing in the unusual form of an ordered 3D domain-swapped supramolecular array. Small angle X-ray scattering measurements show that the self-association properties of trpR in solution are fundamentally altered by isopropanol.
About this StructureAbout this Structure
1MI7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:15274929
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