4iih: Difference between revisions
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==Crystal structure of beta-glucosidase 1 from Aspergillus aculeatus in complex with thiocellobiose== | ==Crystal structure of beta-glucosidase 1 from Aspergillus aculeatus in complex with thiocellobiose== | ||
<StructureSection load='4iih' size='340' side='right' caption='[[4iih]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='4iih' size='340' side='right' caption='[[4iih]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4iih]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4iih]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspac Aspac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IIH FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TCB:THIOCELLOBIOSE'>TCB</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TCB:THIOCELLOBIOSE'>TCB</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4iib|4iib]], [[4iic|4iic]], [[4iid|4iid]], [[4iie|4iie]], [[4iif|4iif]], [[4iig|4iig]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4iib|4iib]], [[4iic|4iic]], [[4iid|4iid]], [[4iie|4iie]], [[4iif|4iif]], [[4iig|4iig]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bgl1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5053 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bgl1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5053 ASPAC])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iih OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iih RCSB], [http://www.ebi.ac.uk/pdbsum/4iih PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iih OCA], [http://pdbe.org/4iih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iih RCSB], [http://www.ebi.ac.uk/pdbsum/4iih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iih ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4iih" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Aspac]] | ||
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
[[Category: Fushinobu, S]] | [[Category: Fushinobu, S]] | ||
Revision as of 14:17, 5 August 2016
Crystal structure of beta-glucosidase 1 from Aspergillus aculeatus in complex with thiocellobioseCrystal structure of beta-glucosidase 1 from Aspergillus aculeatus in complex with thiocellobiose
Structural highlights
Publication Abstract from PubMedGlycoside hydrolase family 3 (GH3) beta-glucosidases (BGLs) from filamentous fungi have been widely and commercially used for supplementation of cellulases. BGL from the fungus Aspergillus aculeatus (AaBGL1) belongs to GH3 and shows high activity toward cellooligosaccharides up to high degree of polymerization. Here we determined the crystal structure of AaBGL1. In addition to the substrate-free structure, complex structures with glucose and various inhibitors were determined. The AaBGL1 structure is highly glycosylated with 88 monosaccharides (18 N-glycan chains) in the dimer. The largest N-glycan chain comprises 10 monosaccharides and is one of the largest glycans ever observed in protein crystal structures. A prominent insertion region exists in a fibronectin type III domain, and this region extends to cover a wide surface area of the enzyme. The subsite +1 of AaBGL1 is highly hydrophobic. Three aromatic residues are involved in the subsite +1 and located in short loop regions that are uniquely present in this enzyme. There is a long cleft extending from the subsite +1, which appears to be suitable for binding long cellooligosaccharides. The crystal structures of AaBGL1 will provide an important structural basis for the technical improvement of enzymatic cellulosic biomass conversion. Crystal Structures of Glycoside Hydrolase Family 3 beta-Glucosidase 1 from Aspergillus aculeatus.,Suzuki K, Sumitani JI, Nam YW, Nishimaki T, Tani S, Wakagi T, Kawaguchi T, Fushinobu S Biochem J. 2013 Mar 28. PMID:23537284[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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