1m4z: Difference between revisions
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|PDB= 1m4z |SIZE=350|CAPTION= <scene name='initialview01'>1m4z</scene>, resolution 2.2Å | |PDB= 1m4z |SIZE=350|CAPTION= <scene name='initialview01'>1m4z</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Orc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= Orc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m4z OCA], [http://www.ebi.ac.uk/pdbsum/1m4z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m4z RCSB]</span> | |||
}} | }} | ||
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[[Category: Xu, R M.]] | [[Category: Xu, R M.]] | ||
[[Category: Zhang, Z.]] | [[Category: Zhang, Z.]] | ||
[[Category: bah domain]] | [[Category: bah domain]] | ||
[[Category: chromatin]] | [[Category: chromatin]] | ||
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[[Category: transcriptional silencing]] | [[Category: transcriptional silencing]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:11:20 2008'' | ||
Revision as of 22:11, 30 March 2008
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| , resolution 2.2Å | |||||||
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| Ligands: | |||||||
| Gene: | Orc1 (Saccharomyces cerevisiae) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the N-terminal BAH domain of Orc1p
OverviewOverview
The N-terminal domain of the largest subunit of the Saccharomyces cerevisiae origin recognition complex (Orc1p) functions in transcriptional silencing and contains a bromo-adjacent homology (BAH) domain found in some chromatin-associated proteins including Sir3p. The 2.2 A crystal structure of the N-terminal domain of Orc1p revealed a BAH core and a non-conserved helical sub-domain. Mutational analyses demonstrated that the helical sub-domain was necessary and sufficient to bind Sir1p, and critical for targeting Sir1p primarily to the cis-acting E silencers at the HMR and HML silent chromatin domains. In the absence of the BAH domain, approximately 14-20% of cells in a population were silenced at the HML locus. Moreover, the distributions of the Sir2p, Sir3p and Sir4p proteins, while normal, were at levels lower than found in wild-type cells. Thus, in the absence of the Orc1p BAH domain, HML resembled silencing of genes adjacent to telomeres. These data are consistent with the view that the Orc1p-Sir1p interaction at the E silencers ensures stable inheritance of pre-established Sir2p, Sir3p and Sir4p complexes at the silent mating type loci.
About this StructureAbout this Structure
1M4Z is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing., Zhang Z, Hayashi MK, Merkel O, Stillman B, Xu RM, EMBO J. 2002 Sep 2;21(17):4600-11. PMID:12198162
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