4ilm: Difference between revisions
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==CRISPR RNA Processing endoribonuclease== | ==CRISPR RNA Processing endoribonuclease== | ||
<StructureSection load='4ilm' size='340' side='right' caption='[[4ilm]], [[Resolution|resolution]] 3.07Å' scene=''> | <StructureSection load='4ilm' size='340' side='right' caption='[[4ilm]], [[Resolution|resolution]] 3.07Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ilm]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4ilm]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ILM FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pkm|3pkm]], [[4ill|4ill]], [[4ilr|4ilr]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pkm|3pkm]], [[4ill|4ill]], [[4ilr|4ilr]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas6b, SSO2004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas6b, SSO2004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ilm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ilm RCSB], [http://www.ebi.ac.uk/pdbsum/4ilm PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ilm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilm OCA], [http://pdbe.org/4ilm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ilm RCSB], [http://www.ebi.ac.uk/pdbsum/4ilm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ilm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ilm" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Endonuclease|Endonuclease]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Sulso]] | ||
[[Category: Li, H]] | [[Category: Li, H]] | ||
[[Category: Shao, Y]] | [[Category: Shao, Y]] | ||
Revision as of 10:08, 5 August 2016
CRISPR RNA Processing endoribonucleaseCRISPR RNA Processing endoribonuclease
Structural highlights
Publication Abstract from PubMedClustered regularly interspaced short palindromic repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no predicted secondary structural features remains largely elusive. Here, we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing. Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6.,Shao Y, Li H Structure. 2013 Feb 27. pii: S0969-2126(13)00017-8. doi:, 10.1016/j.str.2013.01.010. PMID:23454186[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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