1m2v: Difference between revisions
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|PDB= 1m2v |SIZE=350|CAPTION= <scene name='initialview01'>1m2v</scene>, resolution 2.75Å | |PDB= 1m2v |SIZE=350|CAPTION= <scene name='initialview01'>1m2v</scene>, resolution 2.75Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Sec23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Sar1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= Sec23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Sar1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1m2o|1M2O]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m2v OCA], [http://www.ebi.ac.uk/pdbsum/1m2v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m2v RCSB]</span> | |||
}} | }} | ||
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[[Category: Corpina, R A.]] | [[Category: Corpina, R A.]] | ||
[[Category: Goldberg, J.]] | [[Category: Goldberg, J.]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
[[Category: gelsolin domain]] | [[Category: gelsolin domain,]] | ||
[[Category: vwa domain]] | [[Category: vwa domain]] | ||
[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:10:33 2008'' | ||
Revision as of 22:10, 30 March 2008
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| , resolution 2.75Å | |||||||
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| Ligands: | |||||||
| Gene: | Sec23 (Saccharomyces cerevisiae), Sar1 (Saccharomyces cerevisiae) | ||||||
| Related: | 1M2O
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the yeast Sec23/24 heterodimer
OverviewOverview
COPII-coated vesicles form on the endoplasmic reticulum by the stepwise recruitment of three cytosolic components: Sar1-GTP to initiate coat formation, Sec23/24 heterodimer to select SNARE and cargo molecules, and Sec13/31 to induce coat polymerization and membrane deformation. Crystallographic analysis of the Saccharomyces cerevisiae Sec23/24-Sar1 complex reveals a bow-tie-shaped structure, 15 nm long, with a membrane-proximal surface that is concave and positively charged to conform to the size and acidic-phospholipid composition of the COPII vesicle. Sec23 and Sar1 form a continuous surface stabilized by a non-hydrolysable GTP analogue, and Sar1 has rearranged from the GDP conformation to expose amino-terminal residues that will probably embed in the bilayer. The GTPase-activating protein (GAP) activity of Sec23 involves an arginine side chain inserted into the Sar1 active site. These observations establish the structural basis for GTP-dependent recruitment of a vesicular coat complex, and for uncoating through coat-controlled GTP hydrolysis.
About this StructureAbout this Structure
1M2V is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat., Bi X, Corpina RA, Goldberg J, Nature. 2002 Sep 19;419(6904):271-7. PMID:12239560
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