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==The thermo- and acido-stable ORF-99 from the archaeal virus AFV1== | ==The thermo- and acido-stable ORF-99 from the archaeal virus AFV1== | ||
<StructureSection load='3df6' size='340' side='right' caption='[[3df6]], [[Resolution|resolution]] 2.05Å' scene=''> | <StructureSection load='3df6' size='340' side='right' caption='[[3df6]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3df6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3df6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Afv-1 Afv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DF6 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF99 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235266 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF99 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235266 AFV-1])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3df6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3df6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3df6 RCSB], [http://www.ebi.ac.uk/pdbsum/3df6 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3df6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3df6 OCA], [http://pdbe.org/3df6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3df6 RCSB], [http://www.ebi.ac.uk/pdbsum/3df6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3df6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3df6" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Afv-1]] | ||
[[Category: Cambillau, C]] | [[Category: Cambillau, C]] | ||
[[Category: Campanacci, V]] | [[Category: Campanacci, V]] | ||
Revision as of 06:34, 5 August 2016
The thermo- and acido-stable ORF-99 from the archaeal virus AFV1The thermo- and acido-stable ORF-99 from the archaeal virus AFV1
Structural highlights
Publication Abstract from PubMedAcidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 has a new fold, is hyperthermostable (up to 95 degrees C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95 degrees C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues. The thermo- and acido-stable ORF-99 from the archaeal virus AFV1.,Goulet A, Spinelli S, Blangy S, van Tilbeurgh H, Leulliot N, Basta T, Prangishvili D, Cambillau C, Campanacci V Protein Sci. 2009 Jun;18(6):1316-20. PMID:19472363[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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