4mr1: Difference between revisions
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==X-ray structure of the adduct between hen egg white lysozyme and cis-diamminediiodoplatinum(II)== | ==X-ray structure of the adduct between hen egg white lysozyme and cis-diamminediiodoplatinum(II)== | ||
<StructureSection load='4mr1' size='340' side='right' caption='[[4mr1]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='4mr1' size='340' side='right' caption='[[4mr1]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=YPT:CIS-DIAMINO(DIIODO)PLATINUM'>YPT</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=YPT:CIS-DIAMINO(DIIODO)PLATINUM'>YPT</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mr1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mr1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mr1 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mr1 OCA], [http://pdbe.org/4mr1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mr1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mr1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mr1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4mr1" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 06:34, 5 August 2016
X-ray structure of the adduct between hen egg white lysozyme and cis-diamminediiodoplatinum(II)X-ray structure of the adduct between hen egg white lysozyme and cis-diamminediiodoplatinum(II)
Structural highlights
Function[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedThe reactivity of cis-diamminediiodidoplatinum(II), cis-PtI2(NH3)2, the iodo analogue of cisplatin, with hen egg white lysozyme (HEWL) was investigated by electrospray ionization mass spectrometry and X-ray crystallography. Interestingly, the study compound forms a stable 1:1 protein adduct for which the crystal structure was solved at 1.99 A resolution. In this adduct, the Pt(II) center, upon release of one ammonia ligand, selectively coordinates to the imidazole of His15. Both iodide ligands remain bound to platinum, with this being a highly peculiar and unexpected feature. Notably, two equivalent modes of Pt(II) binding are possible that differ only in the location of I atoms with respect to ND1 of His15. The structure of the adduct was compared with that of HEWL-cisplatin, previously described; differences are stressed and their important mechanistic implications discussed. Peculiar features in the crystal structure of the adduct formed between cis-PtI2(NH3)2 and hen egg white lysozyme.,Messori L, Marzo T, Gabbiani C, Valdes AA, Quiroga AG, Merlino A Inorg Chem. 2013 Dec 16;52(24):13827-9. doi: 10.1021/ic402611m. Epub 2013 Nov 20. PMID:24256441[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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