1lba: Difference between revisions
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|PDB= 1lba |SIZE=350|CAPTION= <scene name='initialview01'>1lba</scene>, resolution 2.2Å | |PDB= 1lba |SIZE=350|CAPTION= <scene name='initialview01'>1lba</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span> | ||
|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lba OCA], [http://www.ebi.ac.uk/pdbsum/1lba PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lba RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1LBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1LBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LBA OCA]. | ||
==Reference== | ==Reference== | ||
The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase., Cheng X, Zhang X, Pflugrath JW, Studier FW, Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4034-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8171031 8171031] | The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase., Cheng X, Zhang X, Pflugrath JW, Studier FW, Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4034-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8171031 8171031] | ||
[[Category: | [[Category: Enterobacteria phage t7]] | ||
[[Category: N-acetylmuramoyl-L-alanine amidase]] | [[Category: N-acetylmuramoyl-L-alanine amidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cheng, X.]] | [[Category: Cheng, X.]] | ||
[[Category: hydrolase(acting on linear amides)]] | [[Category: hydrolase(acting on linear amides)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:00:41 2008'' | ||
Revision as of 22:00, 30 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | T7 (Enterobacteria phage T7) | ||||||
| Activity: | N-acetylmuramoyl-L-alanine amidase, with EC number 3.5.1.28 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF BACTERIOPHAGE T7 LYSOZYME, A ZINC AMIDASE AND AN INHIBITOR OF T7 RNA POLYMERASE
OverviewOverview
The lysozyme of bacteriophage T7 is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T7 RNA polymerase. The structure of a mutant T7 lysozyme has been determined by x-ray crystallography and refined at 2.2-A resolution. The protein folds into an alpha/beta-sheet structure that has a prominent cleft. A zinc atom is located in the cleft, bound directly to three amino acids and, through a water molecule, to a fourth. Zinc is required for amidase activity but not for inhibition of T7 RNA polymerase. Alignment of the zinc ligands of T7 lysozyme with those of carboxypeptidase A and thermolysin suggests structural similarity among the catalytic sites for the amidase and these zinc proteases. Mutational analysis identified presumed catalytic residues for amidase activity within the cleft and a surface that appears to be the site of binding to T7 RNA polymerase. Binding of T7 RNA polymerase inhibits amidase activity.
About this StructureAbout this Structure
1LBA is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
ReferenceReference
The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase., Cheng X, Zhang X, Pflugrath JW, Studier FW, Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4034-8. PMID:8171031
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