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==cytochrome c peroxidase in complex with phenol== | ==cytochrome c peroxidase in complex with phenol== | ||
<StructureSection load='4a71' size='340' side='right' caption='[[4a71]], [[Resolution|resolution]] 1.61Å' scene=''> | <StructureSection load='4a71' size='340' side='right' caption='[[4a71]], [[Resolution|resolution]] 1.61Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4a71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4a71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A71 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cpg|1cpg]], [[1bva|1bva]], [[2v23|2v23]], [[3ccx|3ccx]], [[6ccp|6ccp]], [[1aeu|1aeu]], [[1dcc|1dcc]], [[1s6v|1s6v]], [[1bej|1bej]], [[1ml2|1ml2]], [[1ccl|1ccl]], [[1beq|1beq]], [[1aem|1aem]], [[1jci|1jci]], [[1u74|1u74]], [[2pcc|2pcc]], [[2b10|2b10]], [[1aeb|1aeb]], [[1cpd|1cpd]], [[1ccg|1ccg]], [[1cca|1cca]], [[1ac4|1ac4]], [[2y5a|2y5a]], [[1aeo|1aeo]], [[1cmu|1cmu]], [[1aet|1aet]], [[1bep|1bep]], [[1aeh|1aeh]], [[1aev|1aev]], [[2pcb|2pcb]], [[1kxn|1kxn]], [[1aeg|1aeg]], [[1aen|1aen]], [[4ccp|4ccp]], [[5ccp|5ccp]], [[2x07|2x07]], [[2cep|2cep]], [[1cmp|1cmp]], [[1ryc|1ryc]], [[1cce|1cce]], [[2xj8|2xj8]], [[2b0z|2b0z]], [[1dsp|1dsp]], [[1bes|1bes]], [[1z53|1z53]], [[2b12|2b12]], [[1bem|1bem]], [[1mkr|1mkr]], [[1ccj|1ccj]], [[1sog|1sog]], [[1aee|1aee]], [[1cpe|1cpe]], [[1a2f|1a2f]], [[1dse|1dse]], [[1jdr|1jdr]], [[1a2g|1a2g]], [[1aes|1aes]], [[1bek|1bek]], [[1zbz|1zbz]], [[1cck|1cck]], [[1mkq|1mkq]], [[1mk8|1mk8]], [[1ebe|1ebe]], [[1cci|1cci]], [[1krj|1krj]], [[2b11|2b11]], [[1aef|1aef]], [[1cyf|1cyf]], [[1aek|1aek]], [[1ccb|1ccb]], [[1u75|1u75]], [[1ac8|1ac8]], [[1dsg|1dsg]], [[1dj1|1dj1]], [[1dj5|1dj5]], [[2ccp|2ccp]], [[1cmt|1cmt]], [[1aej|1aej]], [[7ccp|7ccp]], [[1aeq|1aeq]], [[1ds4|1ds4]], [[2x08|2x08]], [[1sdq|1sdq]], [[2gb8|2gb8]], [[2xj5|2xj5]], [[1kxm|1kxm]], [[4ccx|4ccx]], [[1kok|1kok]], [[1bj9|1bj9]], [[2bcn|2bcn]], [[1zby|1zby]], [[1ccp|1ccp]], [[2ycg|2ycg]], [[2cyp|2cyp]], [[1dso|1dso]], [[3ccp|3ccp]], [[1stq|1stq]], [[2v2e|2v2e]], [[2xil|2xil]], [[1aa4|1aa4]], [[1aed|1aed]], [[1ccc|1ccc]], [[1cmq|1cmq]], [[1cpf|1cpf]], [[4a6z|4a6z]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cpg|1cpg]], [[1bva|1bva]], [[2v23|2v23]], [[3ccx|3ccx]], [[6ccp|6ccp]], [[1aeu|1aeu]], [[1dcc|1dcc]], [[1s6v|1s6v]], [[1bej|1bej]], [[1ml2|1ml2]], [[1ccl|1ccl]], [[1beq|1beq]], [[1aem|1aem]], [[1jci|1jci]], [[1u74|1u74]], [[2pcc|2pcc]], [[2b10|2b10]], [[1aeb|1aeb]], [[1cpd|1cpd]], [[1ccg|1ccg]], [[1cca|1cca]], [[1ac4|1ac4]], [[2y5a|2y5a]], [[1aeo|1aeo]], [[1cmu|1cmu]], [[1aet|1aet]], [[1bep|1bep]], [[1aeh|1aeh]], [[1aev|1aev]], [[2pcb|2pcb]], [[1kxn|1kxn]], [[1aeg|1aeg]], [[1aen|1aen]], [[4ccp|4ccp]], [[5ccp|5ccp]], [[2x07|2x07]], [[2cep|2cep]], [[1cmp|1cmp]], [[1ryc|1ryc]], [[1cce|1cce]], [[2xj8|2xj8]], [[2b0z|2b0z]], [[1dsp|1dsp]], [[1bes|1bes]], [[1z53|1z53]], [[2b12|2b12]], [[1bem|1bem]], [[1mkr|1mkr]], [[1ccj|1ccj]], [[1sog|1sog]], [[1aee|1aee]], [[1cpe|1cpe]], [[1a2f|1a2f]], [[1dse|1dse]], [[1jdr|1jdr]], [[1a2g|1a2g]], [[1aes|1aes]], [[1bek|1bek]], [[1zbz|1zbz]], [[1cck|1cck]], [[1mkq|1mkq]], [[1mk8|1mk8]], [[1ebe|1ebe]], [[1cci|1cci]], [[1krj|1krj]], [[2b11|2b11]], [[1aef|1aef]], [[1cyf|1cyf]], [[1aek|1aek]], [[1ccb|1ccb]], [[1u75|1u75]], [[1ac8|1ac8]], [[1dsg|1dsg]], [[1dj1|1dj1]], [[1dj5|1dj5]], [[2ccp|2ccp]], [[1cmt|1cmt]], [[1aej|1aej]], [[7ccp|7ccp]], [[1aeq|1aeq]], [[1ds4|1ds4]], [[2x08|2x08]], [[1sdq|1sdq]], [[2gb8|2gb8]], [[2xj5|2xj5]], [[1kxm|1kxm]], [[4ccx|4ccx]], [[1kok|1kok]], [[1bj9|1bj9]], [[2bcn|2bcn]], [[1zby|1zby]], [[1ccp|1ccp]], [[2ycg|2ycg]], [[2cyp|2cyp]], [[1dso|1dso]], [[3ccp|3ccp]], [[1stq|1stq]], [[2v2e|2v2e]], [[2xil|2xil]], [[1aa4|1aa4]], [[1aed|1aed]], [[1ccc|1ccc]], [[1cmq|1cmq]], [[1cpf|1cpf]], [[4a6z|4a6z]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-c_peroxidase Cytochrome-c peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.5 1.11.1.5] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a71 RCSB], [http://www.ebi.ac.uk/pdbsum/4a71 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a71 OCA], [http://pdbe.org/4a71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a71 RCSB], [http://www.ebi.ac.uk/pdbsum/4a71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a71 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4a71" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 18824]] | |||
[[Category: Cytochrome-c peroxidase]] | [[Category: Cytochrome-c peroxidase]] | ||
[[Category: Metcalfe, C L]] | [[Category: Metcalfe, C L]] | ||
[[Category: Moody, P C.E]] | [[Category: Moody, P C.E]] | ||
Revision as of 22:14, 4 August 2016
cytochrome c peroxidase in complex with phenolcytochrome c peroxidase in complex with phenol
Structural highlights
Function[CCPR_YEAST] Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Publication Abstract from PubMedGuaiacol is a universal substrate for all peroxidases, and its use in a simple colorimetric assay has wide applications. However, its exact binding location has never been defined. Here we report the crystal structures of guaiacol bound to cytochrome c peroxidase (CcP). A related structure with phenol bound is also presented. The CcP-guaiacol and CcP-phenol crystal structures show that both guaiacol and phenol bind at sites distinct from the cytochrome c binding site and from the delta-heme edge, which is known to be the binding site for other substrates. Although neither guaiacol nor phenol is seen bound at the delta-heme edge in the crystal structures, inhibition data and mutagenesis strongly suggest that the catalytic binding site for aromatic compounds is the delta-heme edge in CcP. The functional implications of these observations are discussed in terms of our existing understanding of substrate binding in peroxidases [Gumiero A et al. (2010) Arch Biochem Biophys 500, 13-20]. Crystal structure of guaiacol and phenol bound to a heme peroxidase.,Murphy EJ, Metcalfe CL, Nnamchi C, Moody PC, Raven EL FEBS J. 2012 May;279(9):1632-9. doi: 10.1111/j.1742-4658.2011.08425.x. Epub 2011 , Dec 5. PMID:22093282[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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