2x7q: Difference between revisions
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==THE CONSERVED CANDIDA ALBICANS CA3427 GENE PRODUCT DEFINES A NEW FAMILY OF PROTEINS EXHIBITING THE GENERIC PERIPLASMIC BINDING PROTEIN STRUCTURAL FOLD== | ==THE CONSERVED CANDIDA ALBICANS CA3427 GENE PRODUCT DEFINES A NEW FAMILY OF PROTEINS EXHIBITING THE GENERIC PERIPLASMIC BINDING PROTEIN STRUCTURAL FOLD== | ||
<StructureSection load='2x7q' size='340' side='right' caption='[[2x7q]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='2x7q' size='340' side='right' caption='[[2x7q]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2x7q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2x7q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X7Q FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x7p|2x7p]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x7p|2x7p]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x7q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x7q RCSB], [http://www.ebi.ac.uk/pdbsum/2x7q PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x7q OCA], [http://pdbe.org/2x7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x7q RCSB], [http://www.ebi.ac.uk/pdbsum/2x7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x7q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2x7q" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Abergel, C]] | [[Category: Abergel, C]] | ||
[[Category: Claverie, J M]] | [[Category: Claverie, J M]] | ||
Revision as of 20:59, 4 August 2016
THE CONSERVED CANDIDA ALBICANS CA3427 GENE PRODUCT DEFINES A NEW FAMILY OF PROTEINS EXHIBITING THE GENERIC PERIPLASMIC BINDING PROTEIN STRUCTURAL FOLDTHE CONSERVED CANDIDA ALBICANS CA3427 GENE PRODUCT DEFINES A NEW FAMILY OF PROTEINS EXHIBITING THE GENERIC PERIPLASMIC BINDING PROTEIN STRUCTURAL FOLD
Structural highlights
Publication Abstract from PubMedNosocomial diseases due to Candida albicans infections are in constant rise in hospitals, where they cause serious complications to already fragile intensive care patients. Antifungal drug resistance is fast becoming a serious issue due to the emergence of strains resistant to currently available antifungal agents. Thus the urgency to identify new potential protein targets, the function and structure of which may guide the development of new antifungal drugs. In this context, we initiated a comparative genomics study in search of promising protein coding genes among the most conserved ones in reference fungal genomes. The CA3427 gene was selected on the basis of its presence among pathogenic fungi contrasting with its absence in the non pathogenic Saccharomyces cerevisiae. We report the crystal 3D-structure of the Candida albicans CA3427 protein at 2.1 A resolution. The combined analysis of its sequence and structure reveals a structural fold originally associated with periplasmic binding proteins. The CA3427 structure highlights a binding site located between the two protein domains, corresponding to a sequence segment conserved among fungi. Two crystal forms of CA3427 were found, suggesting that the presence or absence of a ligand at the proposed binding site might trigger a "Venus flytrap" motion, coupled to the previously described activity of bacterial periplasmic binding proteins. The conserved binding site defines a new subfamily of periplasmic binding proteins also found in many bacteria of the bacteroidetes division, in a choanoflagellate (a free-living unicellular and colonial flagellate eukaryote) and in a placozoan (the closest multicellular relative of animals). A phylogenetic analysis suggests that this gene family originated in bacteria before its horizontal transfer to an ancestral eukaryote prior to the radiation of fungi. It was then lost by the Saccharomycetales which include Saccharomyces cerevisiae. The Conserved Candida albicans CA3427 Gene Product Defines a New Family of Proteins Exhibiting the Generic Periplasmic Binding Protein Structural Fold.,Santini S, Claverie JM, Mouz N, Rousselle T, Maza C, Monchois V, Abergel C PLoS One. 2011 Apr 11;6(4):e18528. PMID:21494601[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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