4o9r: Difference between revisions
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==Human Smoothened Receptor structure in complex with cyclopamine== | ==Human Smoothened Receptor structure in complex with cyclopamine== | ||
<StructureSection load='4o9r' size='340' side='right' caption='[[4o9r]], [[Resolution|resolution]] 3.20Å' scene=''> | <StructureSection load='4o9r' size='340' side='right' caption='[[4o9r]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4o9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O9R FirstGlance]. <br> | <table><tr><td colspan='2'>[[4o9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O9R FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o9r RCSB], [http://www.ebi.ac.uk/pdbsum/4o9r PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9r OCA], [http://pdbe.org/4o9r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o9r RCSB], [http://www.ebi.ac.uk/pdbsum/4o9r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4o9r" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:29, 4 August 2016
Human Smoothened Receptor structure in complex with cyclopamineHuman Smoothened Receptor structure in complex with cyclopamine
Structural highlights
Function[SMO_HUMAN] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.[1] Publication Abstract from PubMedLipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mum-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor. Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.,Weierstall U, James D, Wang C, White TA, Wang D, Liu W, Spence JC, Bruce Doak R, Nelson G, Fromme P, Fromme R, Grotjohann I, Kupitz C, Zatsepin NA, Liu H, Basu S, Wacker D, Han GW, Katritch V, Boutet S, Messerschmidt M, Williams GJ, Koglin JE, Marvin Seibert M, Klinker M, Gati C, Shoeman RL, Barty A, Chapman HN, Kirian RA, Beyerlein KR, Stevens RC, Li D, Shah ST, Howe N, Caffrey M, Cherezov V Nat Commun. 2014;5:3309. doi: 10.1038/ncomms4309. PMID:24525480[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Bacillus coli migula 1895
- Barty, A
- Basu, S
- Beyerlein, K R
- Boutet, S
- Caffrey, M
- Chapman, H N
- Cherezov, V
- Doak, R B
- Fromme, P
- Fromme, R
- GPCR, GPCR Network
- Gati, C
- Grotjohann, I
- Han, G W
- Howe, N
- James, D
- Katritch, V
- Kirian, R A
- Klinker, M
- Koglin, J E
- Kupitz, C
- Li, D
- Liu, H
- Liu, W
- Messerschmidt, M
- Nelson, G
- Seibert, M M
- Shah, S T.A
- Shoeman, R L
- Spence, J C.H
- Stevens, R C
- Wacker, D
- Wang, C
- Wang, D
- Weierstall, U
- White, T A
- Willams, G J
- Zatsepin, N A
- Cyclopamine
- Free electron laser
- Gpcr
- Gpcr network
- Human smo receptor
- Lcp
- Membrane
- Membrane protein
- Novel protein engineering
- Psi-biology
- Room temperature structure
- Serial femtosecond crystallography
- Signaling protein
- Structural genomic
- Xfel