4fhw: Difference between revisions
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==Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgGTP bound== | ==Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgGTP bound== | ||
<StructureSection load='4fhw' size='340' side='right' caption='[[4fhw]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='4fhw' size='340' side='right' caption='[[4fhw]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4fhw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4fhw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FHW FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fh3|4fh3]], [[4fh5|4fh5]], [[4fhp|4fhp]], [[4fhv|4fhv]], [[4fhx|4fhx]], [[4fhy|4fhy]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fh3|4fh3]], [[4fh5|4fh5]], [[4fhp|4fhp]], [[4fhv|4fhv]], [[4fhx|4fhx]], [[4fhy|4fhy]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cid1, SPAC19D5.03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cid1, SPAC19D5.03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fhw RCSB], [http://www.ebi.ac.uk/pdbsum/4fhw PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fhw OCA], [http://pdbe.org/4fhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fhw RCSB], [http://www.ebi.ac.uk/pdbsum/4fhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fhw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4fhw" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Poly(A) RNA polymerase protein Cid1|Poly(A) RNA polymerase protein Cid1]] | |||
*[[RNA polymerase|RNA polymerase]] | *[[RNA polymerase|RNA polymerase]] | ||
== References == | == References == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Fission yeast]] | ||
[[Category: Lunde, B M]] | [[Category: Lunde, B M]] | ||
[[Category: Magler, I]] | [[Category: Magler, I]] | ||
Revision as of 20:26, 4 August 2016
Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgGTP boundCrystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgGTP bound
Structural highlights
Function[CID1_SCHPO] Involved in cell cycle arrest where in association with crb2/rhp9 and chk1 it inhibits unscheduled mitosis. Publication Abstract from PubMedPolyuridylation is emerging as a ubiquitous post-translational modification with important roles in multiple aspects of RNA metabolism. These poly (U) tails are added by poly (U) polymerases with homology to poly (A) polymerases; nevertheless, the selection for UTP over ATP remains enigmatic. We report the structures of poly (U) polymerase Cid1 from Schizoscaccharomyces pombe alone and in complex with UTP, CTP, GTP and 3'-dATP. These structures reveal that each of the 4 nt can be accommodated at the active site; however, differences exist that suggest how the polymerase selects UTP over the other nucleotides. Furthermore, we find that Cid1 shares a number of common UTP recognition features with the kinetoplastid terminal uridyltransferases. Kinetic analysis of Cid1's activity for its preferred substrates, UTP and ATP, reveal a clear preference for UTP over ATP. Ultimately, we show that a single histidine in the active site plays a pivotal role for poly (U) activity. Notably, this residue is typically replaced by an asparagine residue in Cid1-family poly (A) polymerases. By mutating this histidine to an asparagine residue in Cid1, we diminished Cid1's activity for UTP addition and improved ATP incorporation, supporting that this residue is important for UTP selectivity. Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity.,Lunde BM, Magler I, Meinhart A Nucleic Acids Res. 2012 Aug 9. PMID:22885303[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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