3ayh: Difference between revisions
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==Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III== | ==Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III== | ||
<StructureSection load='3ayh' size='340' side='right' caption='[[3ayh]], [[Resolution|resolution]] 2.19Å' scene=''> | <StructureSection load='3ayh' size='340' side='right' caption='[[3ayh]], [[Resolution|resolution]] 2.19Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ayh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3ayh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AYH FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpc17, SPAPB1E7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpc17, SPAPB1E7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), rpc25, SPBC2G5.07c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ayh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ayh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ayh RCSB], [http://www.ebi.ac.uk/pdbsum/3ayh PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ayh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ayh OCA], [http://pdbe.org/3ayh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ayh RCSB], [http://www.ebi.ac.uk/pdbsum/3ayh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ayh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3ayh" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Fission yeast]] | ||
[[Category: Ehara, H]] | [[Category: Ehara, H]] | ||
[[Category: Sekine, S]] | [[Category: Sekine, S]] | ||
[[Category: Yokoyama, S]] | [[Category: Yokoyama, S]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
Revision as of 17:34, 4 August 2016
Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase IIICrystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III
Structural highlights
Function[RPC9_SCHPO] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.[1] [RPC8_SCHPO] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA.[2] [3] Publication Abstract from PubMedEukaryotic RNA Polymerase III (Pol III) is a multi-subunit enzyme responsible for transcribing tRNA, 5S rRNA, and several small RNAs. Of the 17 subunits in Pol III, the C17 (Rpc17) and C25 (Rpc25) subunits form a stable subcomplex that protrudes from the core polymerase. In the present study, we determined the crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe. The subcomplex adopts an elongated shape, and each subunit has two domains. The two subunits in the subcomplex are tightly packed and extensively interact, with a contact area of 2,080 A(2) . The overall conformation of S. pombe C17/25 is considerably different from the previously reported structure of C17/25 from Saccharomyces cerevisiae, with respect to the position of the C17 HRDC domain, a helix-bundle essential for cell viability. In contrast, the S. pombe C17/25 structure is quite similar to those of the Pol II and archaeal counterparts, Rpb4/7 and RpoE/F, respectively, despite the low sequence similarity. A phylogenetic comparison of the C17 subunits among eukaryotes revealed that they can be classified into three groups, according to the length of the inter-domain linker. S. pombe C17, as well as Rpb4 and RpoF, belongs to the largest group, with the short linker. On the other hand, S. cerevisiae C17 belongs to the smallest group, with the long linker, which probably enables the subcomplex to assume the alternative conformation. Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNA Polymerase III.,Ehara H, Sekine SI, Yokoyama S Protein Sci. 2011 Jun 28. doi: 10.1002/pro.682. PMID:21714024[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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