3ud9: Difference between revisions
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==Crystal Structure Analysis of FGF1-Disaccharide(NI23) complex== | ==Crystal Structure Analysis of FGF1-Disaccharide(NI23) complex== | ||
<StructureSection load='3ud9' size='340' side='right' caption='[[3ud9]], [[Resolution|resolution]] 2.34Å' scene=''> | <StructureSection load='3ud9' size='340' side='right' caption='[[3ud9]], [[Resolution|resolution]] 2.34Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ud9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3ud9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UD9 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=IDY:METHYL+2-O-SULFO-ALPHA-L-IDOPYRANOSIDURONIC+ACID'>IDY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=IDY:METHYL+2-O-SULFO-ALPHA-L-IDOPYRANOSIDURONIC+ACID'>IDY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ud7|3ud7]], [[3ud8|3ud8]], [[3uda|3uda]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ud7|3ud7]], [[3ud8|3ud8]], [[3uda|3uda]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FGF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FGF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ud9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ud9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ud9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ud9 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ud9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ud9 OCA], [http://pdbe.org/3ud9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ud9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ud9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ud9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3ud9" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Human]] | ||
[[Category: Hung, S C]] | [[Category: Hung, S C]] | ||
[[Category: Shi, Z]] | [[Category: Shi, Z]] | ||
[[Category: Heparin/heparan sulfate binding]] | [[Category: Heparin/heparan sulfate binding]] | ||
[[Category: Hormone]] | [[Category: Hormone]] | ||
Revision as of 15:45, 4 August 2016
Crystal Structure Analysis of FGF1-Disaccharide(NI23) complexCrystal Structure Analysis of FGF1-Disaccharide(NI23) complex
Structural highlights
Function[FGF1_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.[1] [2] [3] Publication Abstract from PubMedSeveral biological processes involve glycans, yet understanding their ligand specificities is impeded by their inherent diversity and difficult acquisition. Generating broad synthetic sugar libraries for bioevaluations is a powerful tool in unraveling glycan structural information. In the case of the widely distributed heparan sulfate (HS), however, the 48 theoretical possibilities for its repeating disaccharide call for synthetic approaches that should minimize the effort in an undoubtedly huge undertaking. Here we employed a divergent strategy to afford all 48 HS-based disaccharides from just two orthogonally protected disaccharide precursors. Different combinations and sequence of transformation steps were applied with many downstream intermediates leading up to multiple target products. With the full disaccharide library in hand, affinity screening with fibroblast growth factor-1 (FGF-1) revealed that four of the synthetic sugars bind to FGF-1. The molecular details of the interaction were further clarified through X-ray analysis of the sugar-protein cocrystals. The capability of comprehensive sugar libraries in providing key insights in glycan-ligand interaction is, thus, highlighted. Divergent synthesis of 48 heparan sulfate-based disaccharides and probing the specific sugar-fibroblast growth factor-1 interaction.,Hu YP, Zhong YQ, Chen ZG, Chen CY, Shi Z, Zulueta MM, Ku CC, Lee PY, Wang CC, Hung SC J Am Chem Soc. 2012 Dec 26;134(51):20722-7. doi: 10.1021/ja3090065. Epub 2012 Dec, 14. PMID:23240683[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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