1ksk: Difference between revisions
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|PDB= 1ksk |SIZE=350|CAPTION= <scene name='initialview01'>1ksk</scene>, resolution 2.0Å | |PDB= 1ksk |SIZE=350|CAPTION= <scene name='initialview01'>1ksk</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=URA:URACIL'>URA</scene> | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=URA:URACIL'>URA</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1ksl|1KSL]], [[1ksv|1KSV]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ksk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksk OCA], [http://www.ebi.ac.uk/pdbsum/1ksk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ksk RCSB]</span> | |||
}} | }} | ||
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[[Category: Sivaraman, J.]] | [[Category: Sivaraman, J.]] | ||
[[Category: Stura, E A.]] | [[Category: Stura, E A.]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: pseudouridine synthase]] | [[Category: pseudouridine synthase]] | ||
[[Category: rsua]] | [[Category: rsua]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:52:58 2008'' | ||
Revision as of 21:52, 30 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Pseudouridylate synthase, with EC number 4.2.1.70 | ||||||
| Related: | 1KSL, 1KSV
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF RSUA
OverviewOverview
In Escherichia coli, the pseudouridine synthase RsuA catalyzes formation of pseudouridine (psi) at position 516 in 16S rRNA during assembly of the 30S ribosomal subunit. We have determined the crystal structure of RsuA bound to uracil at 2.0 A resolution and to uridine 5'-monophosphate (UMP) at 2.65 A resolution. RsuA consists of an N-terminal domain connected by an extended linker to the central and C-terminal domains. Uracil and UMP bind in a cleft between the central and C-terminal domains near the catalytic residue Asp 102. The N-terminal domain shows structural similarity to the ribosomal protein S4. Despite only 15% amino acid identity, the other two domains are structurally similar to those of the tRNA-specific psi-synthase TruA, including the position of the catalytic Asp. Our results suggest that all four families of pseudouridine synthases share the same fold of their catalytic domain(s) and uracil-binding site.
About this StructureAbout this Structure
1KSK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP., Sivaraman J, Sauve V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A, Nat Struct Biol. 2002 May;9(5):353-8. PMID:11953756
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