5dvx: Difference between revisions

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-'''Unreleased structure'''
-The entry 5dvx is ON HOLD  until Paper Publication
+==Crystal structure of the catalytic-domain of human carbonic anhydrase IX at 1.6 angstrom resolution==
+<StructureSection load='5dvx' size='340' side='right' caption='[[5dvx]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5dvx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DVX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DVX FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dvx OCA], [http://pdbe.org/5dvx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dvx RCSB], [http://www.ebi.ac.uk/pdbsum/5dvx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dvx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/CAH9_HUMAN CAH9_HUMAN]] Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.<ref>PMID:18703501</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Human carbonic anhydrase IX (hCA IX) expression in many cancers is associated with hypoxic tumors and poor patient outcome. Inhibitors of hCA IX have been used as anti-cancer agents with some entering Phase I clinical trials. hCA IX is a transmembrane protein whose catalytic domain faces the extracellular tumor milieu, which is typically associated with an acidic microenvironment. Here, we show that the catalytic domain of hCA IX (hCA IX-c) exhibits the necessary biochemical/biophysical properties that allow for low pH stability and activity. Furthermore, the unfolding process of hCA IX-c appears to be reversible and its catalytic efficiency is suggested to be correlated directly with its stability between 3.0&lt;pH&lt;8.0 but not at pH&gt;8.0. To rationalize this, we determined the X-ray crystal structure of hCA IX-c to 1.6 A resolution. Insights from this study suggest an understanding of hCA IX-c stability and activity in low pH tumor microenvironments, and may be applicable to determining pH-related effects on other enzymes.
-Authors: Mahon, B.P., Socorro, L., Driscoll, J.M., McKenna, R.
+Structure of carbonic anhydrase IX is adapted for low pH catalysis.,Mahon BP, Bhatt A, Socorro L, Driscoll JM, Okoh C, Lomelino CL, Mboge MY, Kurian JJ, Tu C, Agbandje-McKenna M, Frost SC, McKenna R Biochemistry. 2016 Jul 20. PMID:27439028<ref>PMID:27439028</ref>
-Description: Crystal structure of the catalytic-domain of human carbonic anhydrase IX at 1.6 angstrom resolution
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Driscoll, J.M]]
+<div class="pdbe-citations 5dvx" style="background-color:#fffaf0;"></div>
-[[Category: Mckenna, R]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Carbonate dehydratase]]
+[[Category: Driscoll, J M]]
+[[Category: Mahon, B P]]
+[[Category: McKenna, R]]
 [[Category: Socorro, L]]
-[[Category: Mahon, B.P]]
+[[Category: Carbonic anhydrase ix]]
+[[Category: Catalytic domain]]
+[[Category: Lyase]]
+[[Category: Water network]]